Tang Mi, Zhang Zhidong, Lin Liyuan, Niu Junling, Meng Guangxun, Wang Wei, Wang Jiwen, Wang Yingyan
Department of Neurology, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127 China.
State Key Laboratory of Genetic Engineering, Department of Microbiology, Fudan Microbiome Center, School of Life Sciences, Fudan University, Shanghai, 200438 China.
Phenomics. 2025 Mar 4;5(1):65-75. doi: 10.1007/s43657-025-00228-7. eCollection 2025 Feb.
Although the compositional alterations of gut bacteria in ketogenic diet (KD) have been intensively investigated, the causal relationship between this extreme diet and the microbiota changes is not fully understood. Here, we studied the growth dynamics of intestinal bacteria in KD. We used the CoPTR method to calculate the peak-to-trough ratio (PTR) based on metagenomic sequencing data, serving as an indicator of bacterial growth rates. Notably, , a bacterium strongly linked to the therapeutic benefits of KD, exhibited one of the highest growth rates, aligning with its markedly elevated abundance. Our findings also revealed discrepancies in the change patterns of CoPTR values and relative abundances for various bacteria across different diet groups, some of which might be attributed to the exceptionally high or low growth rates of specific species. For some of the species demonstrating obvious differences in growth rates between KD and standard diet, we conducted in vitro culture experiments, supplementing them with diverse nutritional sources to elucidate the underlying mechanisms. The integrative analysis of bacterial abundance and growth dynamics can help deepen our understanding of the gut microbiota changes caused by KD and the therapeutic effects of this special diet.
The online version contains supplementary material available at 10.1007/s43657-025-00228-7.
尽管对生酮饮食（KD）中肠道细菌的组成变化进行了深入研究，但这种极端饮食与微生物群变化之间的因果关系尚未完全了解。在这里，我们研究了生酮饮食中肠道细菌的生长动态。我们使用CoPTR方法根据宏基因组测序数据计算峰谷比（PTR），作为细菌生长速率的指标。值得注意的是，一种与生酮饮食治疗益处密切相关的细菌表现出最高的生长速率之一，与其显著升高的丰度一致。我们的研究结果还揭示了不同饮食组中各种细菌的CoPTR值和相对丰度变化模式的差异，其中一些可能归因于特定物种异常高或低的生长速率。对于一些在生酮饮食和标准饮食之间生长速率表现出明显差异的物种，我们进行了体外培养实验，为它们补充不同的营养来源以阐明潜在机制。细菌丰度和生长动态的综合分析有助于加深我们对生酮饮食引起的肠道微生物群变化以及这种特殊饮食治疗效果的理解。
在线版本包含可在10.1007/s43657-025-00228-7获取的补充材料。
