Zhuang Lei, Du Lifeng, Liu Haixia, Liu Hongxia, Li Huifang, Zhang Yinhui, Liu Yunchuan, Hou Jian, Li Tian, Yang Delong, Zhang Xueyong, Hao Chenyang
State Key Laboratory of Crop Gene Resources and Breeding/National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Jiaozuo Academy of Agricultural and Forestry Sciences, Jiaozuo, 454000, Henan, China.
Theor Appl Genet. 2025 May 2;138(5):107. doi: 10.1007/s00122-025-04900-4.
Twenty-six QTLs associated with yield-related traits in wheat were identified through joint linkage and association analysis, with TraesCS5A03G0002500 being selected as a candidate gene for QGl.caas-5A.1. As a major staple crop worldwide, continuously increasing wheat yield is crucial for ensuring food security. Wheat yield is influenced by multiple traits, and elucidating the genetic basis of yield-related traits lays a foundation for future gene cloning and molecular mechanism studies. In this study, a recombinant inbred line (RIL) population derived from 292 lines of Hengguan 35/Zhoumai 18 was genotyped with the Affymetrix wheat 660 K SNP array. Combined with the phenotype of the RIL population in 13 environments, linkage analysis of six yield-related traits including plant height, grain number per spike, thousand-grain weight, grain length, grain width, and grain thickness was conducted. A total of 262 quantitative trait locus (QTLs) (logarithm of odds [LOD] > 3) were identified across 21 chromosomes, in which 50 QTLs were repeatedly detected in more than three environments. Numerous QTLs harbored cloned genes and overlapped with those reported in previous studies. Subsequently, joint analysis of genome-wide association study (GWAS) data from the advanced backcross-nested association mapping plus inter-crossed (AB-NAMIC) population and QTLs identified in the RIL population revealed 26 overlapping genomic regions. Notably, the QGl.caas-5A.1 associated with grain length on chromosome 5A was detected in both the RIL and AB-NAMIC populations, and TraesCS5A03G0002500 was selected as a candidate gene. A kompetitive allele-specific PCR (KASP) marker based on a variant [A/G] in TraesCS5A03G0002500 was developed and validated in a natural population containing 350 accessions. Taken together, these results provide valuable information for fine mapping and cloning of yield-related wheat genes in the future.
通过连锁和关联联合分析，鉴定出26个与小麦产量相关性状相关的QTL，其中TraesCS5A03G0002500被选为QGl.caas - 5A.1的候选基因。作为全球主要的主食作物，持续提高小麦产量对于确保粮食安全至关重要。小麦产量受多种性状影响，阐明产量相关性状的遗传基础为未来基因克隆和分子机制研究奠定了基础。在本研究中，利用Affymetrix小麦660K SNP芯片对由292个衡观35/周麦18株系衍生的重组自交系（RIL）群体进行基因分型。结合RIL群体在13个环境中的表型，对株高、每穗粒数、千粒重、粒长、粒宽和粒厚6个产量相关性状进行连锁分析。在21条染色体上共鉴定出262个数量性状位点（QTLs）（对数优势比[LOD]＞3），其中50个QTLs在三个以上环境中被重复检测到。许多QTLs含有已克隆的基因，并且与先前研究报道的基因重叠。随后，对来自高代回交-巢式关联作图加杂交（AB-NAMIC）群体的全基因组关联研究（GWAS）数据与RIL群体中鉴定出的QTLs进行联合分析，揭示了26个重叠的基因组区域。值得注意的是，在RIL和AB-NAMIC群体中均检测到与5A染色体上粒长相关的QGl.caas - 5A.1，TraesCS5A03G0002500被选为候选基因。基于TraesCS5A03G0002500中的一个变异[A/G]开发了一种竞争性等位基因特异性PCR（KASP）标记，并在一个包含350份材料的自然群体中进行了验证。综上所述，这些结果为未来小麦产量相关基因的精细定位和克隆提供了有价值的信息。
