Li Yinglan, Su Jianshuo, Liu Shufan, Li Shuangfeng, Liu Sisi, Zhang Huaizhen, Ding Zhuang, Wang Zhengping, Liu Min, Zhao Yanna
Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng, Shandong 252059, People's Republic of China.
State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha, Hunan 410004, People's Republic of China.
Int J Biol Macromol. 2025 Jun;311(Pt 2):143685. doi: 10.1016/j.ijbiomac.2025.143685. Epub 2025 Apr 30.
In this study, novel surface-modified lycopene liposomes were prepared for functional food applications, with systematic comparison of their physicochemical characteristics and biological evaluation. In contrast to whey protein isolate/polyethylene glycol layer-by-layer assembled lycopene liposomes (Lips-LYC/WPI) and PEGylated lycopene liposomes (LYC Lips), sodium caseinate/polyethylene glycol layer-by-layer assembled lycopene liposomes (Lips-LYC/SC) exhibited significantly reduced particle size and improved stability. Besides, Lips-LYC/SC highlighted enhanced encapsulation efficiency and minimized lycopene leakage attributed to sodium caseinate modification. DSC and PXRD confirmed effective reduction of lycopene crystallinity through excipient interaction, which was conducive to its water solubility improvement. FT-IR and fluorescence analysis revealed intermolecular hydrogen bonding between lycopene and the excipients. Furthermore, DPPH antioxidant and ROS scavenging experiments showed that the encapsulation of lycopene effectively improved its antioxidant activity. Cytotoxicity test revealed that Lips-LYC/SC had minimal cytotoxicity towards LO2 cells and Caco-2 cells, achieving cell survival rates >90 %, while the cell scratch results confirmed that LYC Lips induced significantly slower migration rates towards these cells. Moreover, Lips-LYC/SC significantly ameliorated metabolic disorders, oxidative stress, and hepatotoxicity in HFD-induced liver injury model. The above results highlighted the strategic advantage of sodium caseinate and PEG co-decorated liposomes, establishing Lips-LYC/SC as a promising delivery platform for the hydrophobic bioactive ingredient.
在本研究中，制备了新型表面改性的番茄红素脂质体用于功能性食品应用，并对其理化特性进行了系统比较和生物学评价。与乳清蛋白分离物/聚乙二醇层层组装的番茄红素脂质体（Lips-LYC/WPI）和聚乙二醇化番茄红素脂质体（LYC Lips）相比，酪蛋白酸钠/聚乙二醇层层组装的番茄红素脂质体（Lips-LYC/SC）表现出显著降低的粒径和提高的稳定性。此外，Lips-LYC/SC突出了酪蛋白酸钠修饰导致的封装效率提高和番茄红素泄漏最小化。DSC和PXRD证实通过辅料相互作用有效降低了番茄红素的结晶度，这有利于其水溶性的提高。FT-IR和荧光分析揭示了番茄红素与辅料之间的分子间氢键。此外，DPPH抗氧化和ROS清除实验表明，番茄红素的封装有效地提高了其抗氧化活性。细胞毒性测试表明，Lips-LYC/SC对LO2细胞和Caco-2细胞的细胞毒性最小，细胞存活率>90%，而细胞划痕结果证实LYC Lips诱导这些细胞的迁移速率明显较慢。此外，Lips-LYC/SC显著改善了高脂饮食诱导的肝损伤模型中的代谢紊乱、氧化应激和肝毒性。上述结果突出了酪蛋白酸钠和PEG共修饰脂质体的战略优势，确立了Lips-LYC/SC作为一种有前途的疏水性生物活性成分递送平台。
