Nowroozi Sam, Khashei Varnamkhasti Khalil, Khashei Varnamkhasti Samire, Naeimi Leila, Naeimi Behrouz, Naeimi Sirous
Department of Genetics, College of Science, Kazerun Branch, Islamic Azad University, Kazerun, Iran.
Department of Medical Laboratory Sciences, Faculty of Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran.
BMC Geriatr. 2025 May 3;25(1):307. doi: 10.1186/s12877-025-05978-7.
Oxidative damage is the principal cellular disturbance in the skin aging. Missense polymorphisms strengthen or weaken detoxification enzyme activity. Determination of deleterious functional effects of polymorphisms in detoxification genes (NQO1 and EPHX1) in skin aging was the overall purpose of conducting this hospital-based research.
Cases recruitment on dermatological examination-based evidence performed sequentially between November 2022, and April 2023 at the Motahari Hospital Dermatology Outpatient Clinic. Genotype analysis was performed using PCR-RFLP and T-ARMS -PCR. All statistical analyses were performed using SPSS software, and differences were taken as significant at P < 0.05.
This study results implicate that skin aging obtains on a genetic level and in particular the results suggest that His139Arg, Tyr113His and P187S represent true genetic susceptible loci for cutaneous aging related traits. We found that these new susceptibility loci exhibit sex- and age-specific effect on aging skin risk as well as implicated in interactions with modifiable risk factors including water intake, micronutrient care, sleeping habits, sun exposure and application of sunscreen cream, in the development of an increased risk of aging skin.
Molecular defects associated with the His139Arg, Tyr113His and P187S polymorphisms manifest as an observable change in the external appearance of the skin. This study underscores the need to move toward scrutinizing the ageing skin changes at molecular levels.
氧化损伤是皮肤衰老过程中的主要细胞紊乱现象。错义多态性会增强或削弱解毒酶的活性。本项基于医院的研究的总体目的是确定解毒基因（NQO1和EPHX1）中的多态性对皮肤衰老的有害功能影响。
2022年11月至2023年4月期间，在莫塔哈里医院皮肤科门诊，根据皮肤科检查结果依次招募病例。采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）和等位基因特异性扩增PCR（T-ARMS-PCR）进行基因分型分析。所有统计分析均使用SPSS软件进行，P < 0.05时差异具有统计学意义。
本研究结果表明，皮肤衰老在遗传层面上存在，特别是结果表明，His139Arg、Tyr113His和P187S代表了与皮肤衰老相关特征的真正遗传易感位点。我们发现，这些新的易感位点在衰老皮肤风险方面表现出性别和年龄特异性效应，并且在与可改变的风险因素（包括水分摄入、微量营养素护理、睡眠习惯、阳光照射和涂抹防晒霜）的相互作用中，与皮肤衰老风险增加的发生有关。
与His139Arg、Tyr113His和P187S多态性相关的分子缺陷表现为皮肤外观的可观察到的变化。本研究强调了在分子水平上仔细研究皮肤衰老变化的必要性。
