The human gastrointestinal tract, particularly the colon, is densely populated with microflora, primarily and that are responsible for maintaining diet-based human health. Prebiotics, which are non-living dietary components, selectively stimulate the growth and activity of these beneficial gut microorganisms, offering numerous health benefits. This paper aims to explore the feasibility of utilizing Indonesian exotic fruit seeds as a potential source of prebiotic carbohydrates for functional foods and dietary additives. There are seven Indonesian exotic fruits that have large seed portions and are rich in various carbohydrates that function as prebiotics. These prebiotic carbohydrates, particularly oligosaccharides, can be extracted directly from fruit seeds or produced through enzymatic, fermentation, chemical, or thermal processes, each of which influences the prebiotic composition and effectiveness. Incorporating prebiotic carbohydrates into human health care and functional food production could reduce reliance on synthetic antibiotics. Furthermore, repurposing fruit seed residues for prebiotic production not only contributes to economic growth but also promotes environmental sustainability by minimizing waste.