Urdaneta-Morales S, Tejero F
J Parasitol. 1985 Aug;71(4):409-14.
The Neotropical mammalian parasite Trypanosoma (Herpetosoma) rangeli Tejera, 1920 is difficult to study due to the scarcity of blood forms in the vertebrate host. High and persistent parasitemias (up to 7 times the original inoculum at the peak, and persisting for up to 2 wk) were obtained by i.p. inoculation of infant (6.0 g) male white mice (NMRI strain) with 15 X 10(3) trypomastigotes/g body weight from 12-day-old cultures of the "Dog-82" strain of T. rangeli. This strain was cultured 15 mo at ambient temperature in LIT medium, modified by substituting defibrinated adult rabbit blood for fetal calf serum. These results underlined the importance of host age and time of culture of the parasite as factors influencing levels of parasitemia. The abrupt decline in the parasitemias may be due to an early development of a strong immunological response. Negative xenodiagnoses with Rhodnius prolixus may be due either to sterile immunity in the host mice, or to the low susceptibility of the strain of Rhodnius used. Concurrent experiments established that the T. rangeli strain was not naturally contaminated with T. cruzi.
新热带区哺乳动物寄生虫——1920年特耶拉发现的锥虫（赫佩托体属）兰氏锥虫，由于在脊椎动物宿主体内血内期形态稀缺，难以进行研究。通过给6.0克重的雄性幼龄（NMRI品系）小白鼠腹腔注射每克体重15×10³个来自兰氏锥虫“犬-82”株12日龄培养物的锥鞭毛体，获得了高且持续的寄生虫血症（高峰期可达原始接种量的7倍，并持续长达2周）。该菌株在环境温度下于LIT培养基中培养了15个月，通过用去纤维蛋白的成年兔血替代胎牛血清对培养基进行了改良。这些结果突显了宿主年龄和寄生虫培养时间作为影响寄生虫血症水平因素的重要性。寄生虫血症的突然下降可能是由于早期产生了强烈的免疫反应。用长红猎蝽进行的阴性活体诊断可能是由于宿主小鼠的无菌免疫，或者是所用长红猎蝽品系的低易感性。同时进行的实验证实，兰氏锥虫菌株未被克氏锥虫自然污染。
