孕妇唾液免疫标志物与口腔健康状况之间的关联

Association between Salivary Immune Markers and Oral Health Conditions in Pregnant Women.

作者信息

Alomeir Nora, Mao Xinyue, Yang Ruqian, Assery Nasser, Quataert Sally, Seppo Antti, Lu Xingyi, Wu Tong Tong, Xiao Jin

机构信息

Eastman Institute for Oral Health.

University of Rochester Medical Center.

出版信息

Res Sq. 2025 Apr 14:rs.3.rs-5968375. doi: 10.21203/rs.3.rs-5968375/v1.

DOI:10.21203/rs.3.rs-5968375/v1

PMID:40321779

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12047971/

Abstract

OBJECTIVES

This study aims to assess levels of salivary immune markers in women during the 3rd trimester of pregnancy and explores the association between immune markers and dental caries, as well as oral carriage of oral pathogens and These organisms are known to be associated with oral infectious diseases such as dental caries and oral candida infection.

METHODS

Pregnant women from underserved background participated in this study (n = 181). Participants received a comprehensive oral examination by trained and calibrated dentists. Levels of 36 immune markers in unstimulated saliva samples were measured using Bio-Plex200 (Luminex). Salivary and were quantified using viable count (CFU/ml). Quantities of immune markers and colony-forming-units of and were converted to natural log data for statistical analysis. Latent class analysis was used to assess the clustering effect of immune markers among the participants.

RESULTS

The study found high quantities of salivary immune markers in pregnant women with varying detection rates. Univariate analysis found a higher salivary level of IFN-g and TNF-b among women with < 4 decayed teeth, compared to those with ≥ 4 decayed teeth (p < 0.05). Among women with more than 10 CFU/ml in saliva, FLT-3L, IL-17a, TNF-B, and VEGF-a levels were significantly decreased (p < 0.05), while G-CSF levels were significantly increased (p < 0.05). Lower levels of VEGF-a, Eotaxin and IL-9 were found among the individuals with salivary (p < 0.05). Two distinct clusters were identified among the 181 participants, low-level and high-level of immune markers. The logistic regression model with Least Absolute Shrinkage and Selection Operator (LASSO) penalty identified several potential variables associated with high-level of salivary immune markers. Debiased results indicated statistically significant association between plaque and and immune markers (p < 0.05), with higher levels of these variables correlating with lower level of immune markers. In summary, our research underscores the intricate relationship between caries status, microbial populations, and immune markers in pregnant women.

摘要

目的

本研究旨在评估妊娠晚期妇女唾液免疫标志物水平，并探讨免疫标志物与龋齿、口腔病原体口腔携带之间的关联，这些微生物已知与龋齿和口腔念珠菌感染等口腔传染病有关。

方法

来自服务不足背景的孕妇参与了本研究（n = 181）。参与者接受了由经过培训和校准的牙医进行的全面口腔检查。使用Bio-Plex200（Luminex）测量未刺激唾液样本中36种免疫标志物的水平。唾液中和通过活菌计数（CFU/ml）进行定量。免疫标志物的数量以及和的菌落形成单位转换为自然对数数据进行统计分析。潜在类别分析用于评估参与者中免疫标志物的聚类效应。

结果

研究发现孕妇唾液免疫标志物含量较高，检测率各不相同。单因素分析发现，与有≥4颗龋齿的女性相比，龋齿<4颗的女性唾液中IFN-γ和TNF-β水平更高（p < 0.05）。在唾液中超过10 CFU/ml的女性中，FLT-3L、IL-17a、TNF-B和VEGF-a水平显著降低（p < 0.05），而G-CSF水平显著升高（p < 0.05）。在唾液中有的个体中发现VEGF-a、嗜酸性粒细胞趋化因子和IL-9水平较低（p < 0.05）。在181名参与者中识别出两个不同的聚类，即免疫标志物的低水平和高水平。采用最小绝对收缩和选择算子（LASSO）惩罚的逻辑回归模型确定了几个与唾液免疫标志物高水平相关的潜在变量。去偏结果表明菌斑和与免疫标志物之间存在统计学显著关联（p < 0.05），这些变量水平越高，免疫标志物水平越低。总之，我们的研究强调了孕妇龋齿状况、微生物群和免疫标志物之间的复杂关系。