有睡眠中电持续状态病史患者脑脊液中促炎细胞因子表达增加。
Increased expression of proinflammatory cytokines in the cerebrospinal fluid of patients with a history of electrical status epilepticus in sleep.
作者信息
Ye Xing-Guang, Dong Shi-Wei, Wu Ben-Ze, Liu Zhi-Gang
机构信息
Department of Pediatrics, Foshan Women and Children Hospital, Foshan, 528000, Guangdong, China.
The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510280, Guangdong, China.
出版信息
Neurol Sci. 2025 May 5. doi: 10.1007/s10072-025-08217-4.
OBJECTIVE
This study was to investigate the cytokine expression profiles in the cerebrospinal fluid (CSF) of Self-limited epilepsy with centrotemporal spikes (SeLECTS) patients with electrical status epilepticus during sleep (ESES) and compare these to other neurological diseases to identify the cytokines that correlate with ESES.
METHODS
Seven SeLECTS patients with ESES and nine non-ESES controls were included in this study. CSF was centrifuged within one hour and stored at -80 °C; subsequently, High mobility group box 1 (HMGB1), Caspase-1, interleukin (IL)-1β, IL-1α, IL-6, IL-8, IL-10 and tumour necrosis factor-α (TNF-α) were measured with enzyme-linked immunosorbent assay.
RESULTS
CSF levels of IL-1β, TNF-α, IL-1α, and Caspase-1 in ESES group were significantly elevated compared with those non-ESES controls. Moreover, IL-1β CSF levels were positively correlated with Caspase-1 and TNF-α whereas it moderately correlated with IL-1α.
CONCLUSIONS
The present study indicates a close relationship between neuroinflammation and ESES pathogenesis in patients with SeLECTS.
目的
本研究旨在调查睡眠期癫痫性电持续状态（ESES）的伴中央颞区棘波的自限性癫痫（SeLECTS）患者脑脊液（CSF）中的细胞因子表达谱，并将其与其他神经系统疾病进行比较，以确定与ESES相关的细胞因子。
方法
本研究纳入了7例患有ESES的SeLECTS患者和9例非ESES对照。脑脊液在1小时内离心，并储存在-80°C；随后，采用酶联免疫吸附测定法检测高迁移率族蛋白B1（HMGB1）、半胱天冬酶-1、白细胞介素（IL）-1β、IL-1α、IL-6、IL-·8、IL-10和肿瘤坏死因子-α（TNF-α）。
结果
与非ESES对照相比，ESES组脑脊液中IL-1β、TNF-α、IL-1α和半胱天冬酶-1水平显著升高。此外，脑脊液中IL-1β水平与半胱天冬酶-1和TNF-α呈正相关，而与IL-1α呈中度相关。
结论
本研究表明SeLECTS患者的神经炎症与ESES发病机制之间存在密切关系。
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