Kang Byungjun, Jeong Eunseon, Han Seung Yeop, Heo Jeong Hyun, Lee Yunam, Choi Suah, Choi Yunjung, Kang Donyoung, Hwang Youn-Hoo, Lee Jiin, Seo Jung Hwa, Kim Jinyoung, Jeong Inhea, Kim Enji, Lee Juyoung, Kim Dae-Eun, Park Jang-Ung, Cho Sung-Rae, Jin Yoonhee, Cho Seung-Woo, Lee Hyungsuk
School of Mechanical Engineering, Yonsei University, Seoul, 03722, Republic of Korea.
Department of Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea.
Nat Commun. 2025 May 5;16(1):4174. doi: 10.1038/s41467-025-59026-4.
To build in vitro tissues for therapeutic applications, it is essential to replicate the spatial distribution of cells that occurs during morphogenesis in vivo. However, it remains technically challenging to simultaneously regulate the geometric alignment and aggregation of cells during tissue fabrication. Here, we introduce the acoustofluidic bioassembly induced morphogenesis, which is the combination of precise arrangement of cells by the mechanical forces produced by acoustofluidic cues, and the morphological and functional changes of cells in the following in vitro and in vivo cultures. The acoustofluidic bioassembly can be used to create tissues with regulated nano-, micro-, and macro-structures. We demonstrate that the neuromuscular tissue fabricated with the acoustofluidic bioassembly exhibits enhanced contraction dynamics, electrophysiology, and therapeutic efficacy. The potential of the acoustofluidic bioassembly as an in situ application is demonstrated by fabricating artificial tissues at the defect sites of living tissues. The acoustofluidic bioassembly induced morphogenesis can provide a pioneering platform to fabricate tissues for biomedical applications.
为构建用于治疗应用的体外组织，复制体内形态发生过程中细胞的空间分布至关重要。然而，在组织制造过程中同时调节细胞的几何排列和聚集在技术上仍然具有挑战性。在此，我们引入声流体生物组装诱导形态发生，它是由声流体线索产生的机械力对细胞进行精确排列，以及随后在体外和体内培养中细胞的形态和功能变化的结合。声流体生物组装可用于创建具有可控纳米、微米和宏观结构的组织。我们证明，用声流体生物组装制造的神经肌肉组织表现出增强的收缩动力学、电生理学和治疗效果。通过在活组织的缺损部位制造人工组织，证明了声流体生物组装作为原位应用的潜力。声流体生物组装诱导形态发生可为制造用于生物医学应用的组织提供一个开创性平台。
