重新构想即时检验：用于多重体外诊断的自动化数字微流控技术。

Reimagining point-of-care assays: Automated digital microfluidics for multiplex in vitro diagnostics.

作者信息

Fan Wenkai, Li Donghao, Ding Jingyi, Li Zida

机构信息

Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China.

出版信息

Talanta. 2025 Nov 1;294:128270. doi: 10.1016/j.talanta.2025.128270. Epub 2025 May 3.

DOI:10.1016/j.talanta.2025.128270

PMID:40327981

Abstract

In vitro diagnostics (IVD) is critical in disease screening and diagnosis. Point-of-care testing (POCT), which miniaturizes the IVD tests into portable chips and analyzers, offers the opportunity to decentralize the tests and obtain assay results on-site. Digital microfluidics (DMF) holds the merits of a small footprint, high automation, and low cost, making it a powerful tool for implementing various IVD assays for POCT settings. This review aims to consolidate the applications of DMF technology in different POCT settings and provide insights for DMF-based assay development. We first present the mechanism and fabrication processes of DMF technology. We then present its specific POCT applications, including infectious disease monitoring, neonatal disease screening, self-testing of chronic diseases, and routine disease screening. We further discuss the challenges in the development of DMF-based POCT technologies and provide an outlook on this exciting field.

摘要

体外诊断（IVD）在疾病筛查和诊断中至关重要。即时检测（POCT）将IVD检测小型化为便携式芯片和分析仪，为分散检测并在现场获得检测结果提供了机会。数字微流控（DMF）具有占地面积小、自动化程度高和成本低的优点，使其成为在POCT环境中实施各种IVD检测的有力工具。本综述旨在巩固DMF技术在不同POCT环境中的应用，并为基于DMF的检测开发提供见解。我们首先介绍DMF技术的机制和制造工艺。然后介绍其在POCT中的具体应用，包括传染病监测、新生儿疾病筛查、慢性病自我检测和常规疾病筛查。我们进一步讨论基于DMF的POCT技术开发中的挑战，并对这一令人兴奋的领域进行展望。