Margariti Myrto, Roussaki Marianna, Sakellis Elias, Boukos Nikos, Theodosiou Maria, Kostopoulou Nikoleta, Efthimiadou Eleni K
Inorganic Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Zografou, 15771, Greece.
Institute of Nanoscience and Nanotechnology, National Center of Scientific Research "Demokritos", Agia Paraskevi, 15341, Greece.
Chemistry. 2025 Jul 17;31(40):e202500986. doi: 10.1002/chem.202500986. Epub 2025 May 24.
A dextran templated biomimetic synthetic approach was applied for the preparation of Cadmium Selinium:Europium Quantum Dots (CdSe:Eu QDs). The present work examines synthetic variables including the effect of increasing Europium concentration during QDs' synthesis and the use of different coating agents (citrate versus APTES) to improve the QDs' colloidal stability and ameliorate their cytotoxic behavior. All samples were subjected to structural and morphological characterizations via Fourier Transform infrared (FT-IR) spectroscopy, UV-Visible (UV-Vis) spectrophotometry, Transmission Electron Microscopy (TEM), and Dynamic Light Scattering (DLS). Subsequently, biological evaluation of all the synthesized samples of bare and APTES coated CdSe:Eu QDs was conducted on epithelial HaCaT and breast cancer MCF-7. Wound healing assays were conducted to assess cytotoxicity and cell migration under QD treatment. Results showed that both coated and uncoated QDs influenced wound closure rates in a concentration- and time-dependent manner. Finally, fluorescence microscopy revealed significant green luminescence of CdSe:Eu quantum dots (QDs) in both HaCaT and MCF-7 cell lines, indicating efficient cellular uptake. The internalization of QDs was influenced by their surface properties and charge, suggesting an endocytic uptake pathway.
铕量子点（CdSe:Eu QDs）。本研究考察了合成变量，包括量子点合成过程中铕浓度增加的影响以及使用不同的包覆剂（柠檬酸盐与APTES）来提高量子点的胶体稳定性并改善其细胞毒性行为。所有样品均通过傅里叶变换红外（FT-IR）光谱、紫外可见（UV-Vis）分光光度法、透射电子显微镜（TEM）和动态光散射（DLS）进行结构和形态表征。随后，对裸露的和APTES包覆的CdSe:Eu QDs的所有合成样品在上皮HaCaT细胞和乳腺癌MCF-7细胞上进行了生物学评价。进行伤口愈合试验以评估量子点处理下的细胞毒性和细胞迁移。结果表明，包覆和未包覆的量子点均以浓度和时间依赖性方式影响伤口闭合率。最后，荧光显微镜显示CdSe:Eu量子点（QDs）在HaCaT和MCF-7细胞系中均有显著的绿色荧光，表明细胞摄取效率高。量子点的内化受其表面性质和电荷的影响，提示内吞摄取途径。
