Hitch Thomas C A, Masson Johannes M, Pauvert Charlie, Bosch Johanna, Nüchtern Selina, Treichel Nicole S, Baloh Marko, Razavi Soheila, Afrizal Afrizal, Kousetzi Ntana, Aguirre Andrea M, Wylensek David, Coates Amy C, Jennings Susan A V, Panyot Atscharah, Viehof Alina, Schmitz Matthias A, Stuhrmann Maximilian, Deis Evelyn C, Bisdorf Kevin, Chiotelli Maria D, Lissin Artur, Schober Isabel, Witte Julius, Cramer Thorsten, Riedel Thomas, Wende Marie, Winter Katrin A, Amend Lena, Riva Alessandra, Trinh Stefanie, Mitchell Laura, Hartman Jonathan, Berry David, Seitz Jochen, Bossert Lukas C, Grognot Marianne, Allers Thorsten, Strowig Till, Pester Michael, Abt Birte, Reimer Lorenz C, Overmann Jörg, Clavel Thomas
Functional Microbiome Research Group, Institute of Medical Microbiology, University Hospital of RWTH Aachen, Aachen, Germany.
Biophysics of Host-Microbe Interactions Research Group, Institute of Medical Microbiology, University Hospital of RWTH Aachen, Aachen, Germany.
Nat Commun. 2025 May 6;16(1):4203. doi: 10.1038/s41467-025-59229-9.
Numerous bacteria in the human gut microbiome remain unknown and/or have yet to be cultured. While collections of human gut bacteria have been published, few strains are accessible to the scientific community. We have therefore created a publicly available collection of bacterial strains isolated from the human gut. The Human intestinal Bacteria Collection (HiBC) ( https://www.hibc.rwth-aachen.de ) contains 340 strains representing 198 species within 29 families and 7 phyla, of which 29 previously unknown species are taxonomically described and named. These included two butyrate-producing species of Faecalibacterium and new dominant species associated with health and inflammatory bowel disease, Ruminococcoides intestinale and Blautia intestinihominis, respectively. Plasmids were prolific within the HiBC isolates, with almost half (46%) of strains containing plasmids, with a maximum of six within a strain. This included a broadly occurring plasmid (pBAC) that exists in three diverse forms across Bacteroidales species. Megaplasmids were identified within two strains, the pMMCAT megaplasmid is globally present within multiple Bacteroidales species. This collection of easily searchable and publicly available gut bacterial isolates will facilitate functional studies of the gut microbiome.
人类肠道微生物群中的许多细菌仍然未知且/或尚未培养出来。虽然已发表了人类肠道细菌的集合，但科学界能够获取的菌株很少。因此，我们创建了一个可公开获取的从人类肠道分离的细菌菌株集合。人类肠道细菌集合（HiBC）（https://www.hibc.rwth-aachen.de ）包含340个菌株，代表29个科和7个门中的198个物种，其中29个先前未知的物种在分类学上得到了描述和命名。这些包括两种产生丁酸盐的粪杆菌属物种，以及分别与健康和炎症性肠病相关的新的优势物种，即肠道瘤胃球菌和肠道人拟杆菌。质粒在HiBC分离株中很常见，几乎一半（46%）的菌株含有质粒，一个菌株中最多有六个质粒。这包括一种广泛存在的质粒（pBAC），它以三种不同形式存在于拟杆菌目物种中。在两个菌株中鉴定出了大质粒，pMMCAT大质粒在多个拟杆菌目物种中普遍存在。这个易于搜索且可公开获取的肠道细菌分离株集合将有助于肠道微生物群的功能研究。
