Khan Shumaila, Iqbal Muhammad Zafar, Solangi Farheen, Azeem Shahid, Bodlah Muhammad Adnan, Zaheer Muhammad Saqlain, Niaz Yasir, Ashraf Muhammad, Abid Muhammad, Gul Hera, Yu Hongjun, Li Qiang, Weijie Jiang, Rizwan Muhammad, Manoharadas Salim
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences (CAAS), No.12 Zhongguancun South Street, Haidian District, Beijing, 100081, China.
Climate Smart Agriculture Accelerator Program (CSAAP) KFUEIT GIZ SAR, Rahim Yar Khan, Pakistan.
Sci Rep. 2025 May 6;15(1):15829. doi: 10.1038/s41598-025-00294-x.
Lettuce (Lactuca sativa L.), a widely cultivated leafy green, is valued for its rich content of bioactive compounds, including folates, vitamins, tocopherols, ascorbic acid, and antioxidants. This study aimed to evaluate the effects of amino acid supplementation on the growth and nutrient content of hydroponically grown lettuce. A greenhouse experiment using a completely randomized design (CRD) was conducted, with three replications and three plants per replication. There were 4 treatments (T (Control), T (Methionine 20 mg/L), T (Tryptophan 220 mg/L, T (Glycine 200 mg/L) of this experiment Growth parameters, including biomass, leaf length, leaf width, and leaf area, were measured four weeks after transplantation. L-methionine supplementation resulted in a significant improvement in plant growth, with a 23.60% increase in biomass and a 31.41% increase in leaf area. Conversely, L-tryptophan treatment led to substantial reductions in growth, including a 98.78% decrease in biomass. Nutrient analysis revealed that amino acid treatments, especially methionine, enhanced the nitrogen, phosphorus, and potassium content in leaf tissues. These results suggest that L-methionine has a positive effect on both growth and nutrient uptake in hydroponic lettuce, while L-tryptophan and L-glycine negatively affect plant development. The differential responses to amino acid treatments may be attributed to their distinct roles in plant metabolism, with methionine enhancing sulfur-containing compounds and proteins essential for growth, while tryptophan and glycine could disrupt metabolic pathways. Future research should explore the mechanisms underlying these effects and evaluate the optimal amino acid concentrations for maximizing hydroponic lettuce production and nutrient density.
生菜（Lactuca sativa L.）是一种广泛种植的绿叶蔬菜，因其富含生物活性化合物而受到重视，这些化合物包括叶酸、维生素、生育酚、抗坏血酸和抗氧化剂。本研究旨在评估氨基酸添加对水培生菜生长和营养成分的影响。采用完全随机设计（CRD）进行温室试验，重复3次，每次重复3株植株。本试验有4种处理（T（对照）、T（蛋氨酸20mg/L）、T（色氨酸220mg/L）、T（甘氨酸200mg/L））。在移栽4周后测量生长参数，包括生物量、叶长、叶宽和叶面积。添加L-蛋氨酸显著促进了植株生长，生物量增加了23.60%，叶面积增加了31.41%。相反，L-色氨酸处理导致生长大幅下降，包括生物量下降98.78%。营养分析表明，氨基酸处理，尤其是蛋氨酸，提高了叶片组织中的氮、磷和钾含量。这些结果表明，L-蛋氨酸对水培生菜的生长和养分吸收具有积极作用，而L-色氨酸和L-甘氨酸对植株发育具有负面影响。对氨基酸处理的不同反应可能归因于它们在植物代谢中的不同作用，蛋氨酸可增强生长必需的含硫化合物和蛋白质，而色氨酸和甘氨酸可能扰乱代谢途径。未来的研究应探索这些影响的潜在机制，并评估使水培生菜产量和营养密度最大化的最佳氨基酸浓度。
