Hao Guifeng, Chen Qing, Liu Ying, Wu Chunling, An Xingkui, Gregory Ijiti Oluwole, Liang Xiao
School of Tropical Agriculture and Forestry, Hainan University, Haikou, 571000, China.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.
Exp Appl Acarol. 2025 May 6;94(4):53. doi: 10.1007/s10493-025-01020-y.
Uridine diphosphate (UDP)-glycosyltransferase (UGT) belongs to detoxification enzyme glycosylating lipophilic xenobiotic compounds in various living organisms. Tetranychus urticae is a notorious pest due to its significant threat to crop production and serious resistance problem worldwide. However, the function of UGT gene in contributing to pesticide resistance in T. urticae remained largely unknown. In this study, it was found that the laboratory selected abamectin-resistant (AbR) strain had developed over 20,000-fold resistance compared with the susceptible strain (SS). After being treated with abamectin, the activities of UGTs, and the transcription of TuUGT201D3 in the AbR strain were significantly higher than those in SS. Molecular docking indicated that the UGT201D3 protein exhibited high binding capacity with abamectin, suggesting the potential interaction between them. Furthermore, knock-down the transcription of TuUGT201D3 led to the decrease of activities of UGTs, in addition, the mortalities of AbR strain (58.4%) will significantly increase compared to control (41.1%) under 48 h of abamectin treatment. Those findings elucidated that TuUGT201D3 was correlated with abamectin resistance in T. urticae.
尿苷二磷酸（UDP）-糖基转移酶（UGT）属于解毒酶，可对各种生物体中的亲脂性外源性化合物进行糖基化修饰。由于对全球作物生产构成重大威胁且存在严重的抗性问题，二斑叶螨是一种臭名昭著的害虫。然而，UGT基因在二斑叶螨对杀虫剂抗性中的作用在很大程度上仍不清楚。在本研究中，发现实验室选育的阿维菌素抗性（AbR）品系与敏感品系（SS）相比已产生了超过20000倍的抗性。用阿维菌素处理后，AbR品系中UGT的活性以及TuUGT201D3的转录水平均显著高于SS品系。分子对接表明，UGT201D3蛋白与阿维菌素有高结合能力，提示它们之间可能存在相互作用。此外，敲低TuUGT201D3的转录会导致UGT活性降低，而且在阿维菌素处理48小时后，AbR品系的死亡率（58.4%）与对照（41.1%）相比将显著增加。这些发现阐明了TuUGT201D3与二斑叶螨对阿维菌素的抗性相关。
