Cong Lin, Li Si-Chen, Wei Zhi-Tang, Yu Shi-Jiang, Huang Ze-Hao, Cui Yang-Yang, Yang Qi-Qi, Ding Li-Li, Pan Qi, Liu Liu, Li Yang, Ran Chun
Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing, China.
College of Horticulture, Sichuan Agricultural University, Chengdu, China.
Pest Manag Sci. 2025 Jun;81(6):3212-3219. doi: 10.1002/ps.8691. Epub 2025 Jan 31.
Cyetpyrafen is a newly developed acaricide. The citrus red mite, Panonychus citri (McGregor), is an important citrus pest that has developed resistance to cyetpyrafen. Uridine diphosphate-glycosyltransferases (UGTs) have been widely reported to be associated with resistance to multiple acaricides. However, it has been rarely documented that UGT genes participate in cyetpyrafen resistance in P. citri.
In this study, a significantly upregulated UGT gene, PcUGT201E1, was identified in P. citri using transcriptome analysis. Expression of PcUGT201E1 was significantly upregulated at all stages in the cyetpyrafen-resistant strain and silencing PcUGT201E1 significantly increased the susceptibility of P. citri to cyetpyrafen. Molecular docking of PcUGT201E1 with uridine diphosphate glucose (UDPG) and cyetpyrafen indicated that UDPG and cyetpyrafen can interact with PcUGT202E1 via hydrogen bonds. Heterologous expression and in vitro functional assays revealed that enzyme activity could be inhibited by cyetpyrafen and that recombinant PcUGT201E1 can deplete cyetpyrafen.
These results indicated that PcUGT201E1 participates in cyetpyrafen resistance in P. citri by sequestration, and provided a molecular foundation for understanding cyetpyrafen resistance in P. citri. © 2025 Society of Chemical Industry.
乙螨唑是一种新开发的杀螨剂。柑橘全爪螨（Panonychus citri (McGregor)）是一种重要的柑橘害虫，已对乙螨唑产生抗性。尿苷二磷酸糖基转移酶（UGTs）已被广泛报道与对多种杀螨剂的抗性有关。然而，鲜有文献记载UGT基因参与柑橘全爪螨对乙螨唑的抗性。
在本研究中，通过转录组分析在柑橘全爪螨中鉴定出一个显著上调的UGT基因PcUGT201E1。在乙螨唑抗性品系的各个发育阶段，PcUGT201E1的表达均显著上调，沉默PcUGT201E1可显著提高柑橘全爪螨对乙螨唑的敏感性。PcUGT201E1与尿苷二磷酸葡萄糖（UDPG）和乙螨唑的分子对接表明，UDPG和乙螨唑可通过氢键与PcUGT202E1相互作用。异源表达和体外功能测定表明，乙螨唑可抑制酶活性，重组PcUGT201E1可消耗乙螨唑。
这些结果表明，PcUGT201E1通过隔离作用参与柑橘全爪螨对乙螨唑的抗性，为理解柑橘全爪螨对乙螨唑的抗性提供了分子基础。© 2025化学工业协会。
