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一种用于检测糖尿病生物标志物羧甲基赖氨酸的免疫传感器。

An immunosensor for the detection of -(carboxymethyl)lysine - a diabetic biomarker.

作者信息

Kumar Priyanga, Nesakumar Noel, Vedantham Srinivasan, Balaguru Rayappan John Bosco

机构信息

Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA Deemed University Thanjavur Tamil Nadu 613 401 India

School of Electrical & Electronics Engineering (SEEE), SASTRA Deemed University Thanjavur 613 401 India.

出版信息

RSC Adv. 2025 May 6;15(18):14375-14384. doi: 10.1039/d5ra00968e. eCollection 2025 Apr 28.

DOI:10.1039/d5ra00968e
PMID:40330040
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12053838/
Abstract

Carboxymethyl-lysine (CML) is a well-known lysine product that strongly correlates with type 2 diabetes mellitus (T2DM), and its elevated levels are significantly associated with renal impairment and T2DM-related complications. Thus, it is imperative to quantify CML levels and recognize the onset of hyperglycemia and its consequences. In this context, the development of an electrochemical immunosensor for the rapid and ultralow-level detection of CML was attempted. The fabrication of the working electrode involves the covalent immobilization of anti-CML/EDC-NHS on the surface of a carbon quantum dot (CQD)-modified glassy carbon electrode (GCE). The immunosensor exhibited two discrete linear concentration ranges of 0.5-5.0 ng mL and 5.5-10.0 ng mL, with limits of detection and quantification of 0.027 and 0.087 ng mL and 0.16 and 0.51 ng mL, respectively. The observed specificity and other merits of the sensor make it suitable for testing human plasma samples.

摘要

羧甲基赖氨酸（CML）是一种著名的赖氨酸产物，与2型糖尿病（T2DM）密切相关，其水平升高与肾功能损害及T2DM相关并发症显著相关。因此，量化CML水平并识别高血糖的发生及其后果至关重要。在此背景下，尝试开发一种用于快速、超微量检测CML的电化学免疫传感器。工作电极的制备包括将抗CML/EDC-NHS共价固定在碳量子点（CQD）修饰的玻碳电极（GCE）表面。该免疫传感器表现出两个离散的线性浓度范围，分别为0.5 - 5.0 ng/mL和5.5 - 10.0 ng/mL，检测限和定量限分别为0.027和0.087 ng/mL以及0.16和0.51 ng/mL。该传感器所观察到的特异性和其他优点使其适用于检测人体血浆样本。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2266/12053838/4eebce181b3c/d5ra00968e-f8.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2266/12053838/85e911deef18/d5ra00968e-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2266/12053838/4eebce181b3c/d5ra00968e-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2266/12053838/285f05aaa2da/d5ra00968e-s1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2266/12053838/4eebce181b3c/d5ra00968e-f8.jpg

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本文引用的文献

1
Circulating Concentrations of advanced Glycation end Products, Carboxymethyl Lysine and Methylglyoxal are Associated With Renal Function in Individuals With Diabetes.在糖尿病患者中，循环中晚期糖基化终产物、羧甲基赖氨酸和甲基乙二醛的浓度与肾功能相关。
J Ren Nutr. 2024 Mar;34(2):154-160. doi: 10.1053/j.jrn.2023.09.005. Epub 2023 Oct 5.
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A new generation of sensors for non-invasive blood glucose monitoring.新一代用于无创血糖监测的传感器。
Am J Transl Res. 2023 Jun 15;15(6):3825-3837. eCollection 2023.
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Advanced Glycation End Products: A Comprehensive Review of Their Detection and Occurrence in Food.
晚期糖基化终产物：关于其在食品中的检测与存在情况的全面综述
Foods. 2023 May 23;12(11):2103. doi: 10.3390/foods12112103.
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Wearable Electrochemical Glucose Sensors in Diabetes Management: A Comprehensive Review.可穿戴电化学葡萄糖传感器在糖尿病管理中的应用：全面综述。
Chem Rev. 2023 Jun 28;123(12):7854-7889. doi: 10.1021/acs.chemrev.3c00078. Epub 2023 May 30.
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N-(carboxymethyl)lysine promotes lipid uptake of macrophage cluster of differentiation 36 and receptor for advanced glycation end products.N-（羧甲基）赖氨酸促进巨噬细胞分化簇36和晚期糖基化终产物受体的脂质摄取。
World J Diabetes. 2023 Mar 15;14(3):222-233. doi: 10.4239/wjd.v14.i3.222.
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Optical Glucose Sensors Based on Chitosan-Capped ZnS-Doped Mn Nanomaterials.基于壳聚糖封端的 ZnS 掺杂 Mn 纳米材料的光学葡萄糖传感器。
Sensors (Basel). 2023 Mar 6;23(5):2841. doi: 10.3390/s23052841.
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N -(carboxymethyl)lysine in bakery products: A review.烘焙产品中的 N -（羧甲基）赖氨酸：综述。
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