Adibi Meysam, Javanmardi Kazem, Saeed Noor Al-Huda Ali A H, Ali Mobasher Mohammad, Jokar Javad, Ghasemian Abdolmajid, Rahimian Niloofar, Hekmat Ava Soltani
Department of Biotechnology, Faculty of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Department of Physiology, Faculty of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Iran J Microbiol. 2025 Feb;17(1):69-79. doi: 10.18502/ijm.v17i1.17803.
and are the two leading bacterial strains involved in wound infections. These bacteria have developed broad resistance to antibiotics, which has complicated their eradication. Additionally, the formation of a polymicrobial infection poses additional problems. Among alternative or complementary options, bacteriophages, viruses that parasitize bacterial hosts, have been promising.
In this research work, bacteriophages' therapeutic effects against - and -infected burn wounds were studied. The infectious burn wound model was performed on Balb/C male mice, aged six weeks and weighing 25 ± 5 gr. The effects of the Hydroxy-propyl-methyl cellulose (HPMC) gel containing phage were investigated compared to gentamicin. All of these actions were performed in separate groups for each bacteria and mixed group of bacteria (to test multi-bacterial infections treating) and the result were compared.
Phages appear to be effective in gel forms. Pathologic samples of different groups confirmed therapeutic results of phages. These results at the microscopic level indicated the recovery of the tissue and the removal of the infection.
The results of this study indicate that lytic phages are powerful biological tools for the treatment of bacterial infections in burn wounds, which can be considered as one of the alternatives for drug-resistant bacterial species and the high costs of antibiotics; though further animal and trial studies are needed. Meanwhile, the complications due to their widespread use in humans should be investigated in more details.
[细菌名称1]和[细菌名称2]是导致伤口感染的两种主要细菌菌株。这些细菌已对多种抗生素产生广泛耐药性，这使得根除它们变得复杂。此外，混合菌感染的形成带来了更多问题。在替代或补充治疗方案中，噬菌体（寄生于细菌宿主的病毒）已显示出前景。
在本研究中，研究了噬菌体对[细菌名称1]和[细菌名称2]感染的烧伤创面的治疗效果。在六周龄、体重25±5克的雄性Balb/C小鼠上建立感染性烧伤创面模型。将含噬菌体的羟丙基甲基纤维素（HPMC）凝胶与庆大霉素的效果进行比较研究。所有这些操作针对每种细菌分别分组进行，并设置细菌混合组（以测试对混合菌感染的治疗效果），然后比较结果。
噬菌体以凝胶形式似乎具有疗效。不同组的病理样本证实了噬菌体的治疗效果。这些微观层面的结果表明组织得以恢复且感染被清除。
本研究结果表明，裂解性噬菌体是治疗烧伤创面细菌感染的有力生物工具，可被视为耐药细菌种类及抗生素高昂成本问题的替代方案之一；不过还需要进一步的动物和试验研究。同时，应更详细地研究其在人类广泛使用可能导致的并发症。
