Anthem Biosciences Pvt Ltd., Bangalore, Karnataka, India.
Antibiotic Resistance and Phage Therapy Laboratory, Vellore Institute of Technology, School of Biosciences and Technology, Vellore, Tamil Nadu, 632014, India.
BMC Microbiol. 2022 Aug 1;22(1):187. doi: 10.1186/s12866-022-02603-0.
Pseudomonas aeruginosa is an opportunistic pathogen that can cause a variety of infections in humans, such as burn wound infections and infections of the lungs, the bloodstream and surgical site infections. Nosocomial spread is often concurrent with high degrees of antibiotic resistance. Such resistant strains are difficult to treat, and in some cases, even reserved antibiotics are ineffective. A particularly promising therapy to combat infections of resistant bacteria is the deployment of bacteriophages, known as phage therapy. In this work, we evaluated the in vivo efficacy of two Pseudomonas phages in bacteremia mice models. For this study, non-neutropenic mice (BalB/C) were infected with P. aeruginosa AB030 strain and treated using two bacteriophages, AP025 and AP006.
The results showed that a single dose of phages at higher concentrations, bacteria: phage at 1:10 and 1:100 were effective in eliminating the bloodstream infection and achieving 100% mice survival.
This study highlights the efficacy of using a single dose of phages to restore mice from bacteremia.
铜绿假单胞菌是一种机会致病菌，可引起人类多种感染，如烧伤创面感染、肺部感染、血流感染和外科部位感染。医院感染通常与高度抗生素耐药性同时发生。这种耐药菌株难以治疗，在某些情况下，即使保留抗生素也无效。一种特别有前途的治疗耐药菌感染的方法是部署噬菌体，称为噬菌体疗法。在这项工作中，我们评估了两种铜绿假单胞菌噬菌体在菌血症小鼠模型中的体内疗效。在这项研究中，非中性粒细胞减少（BalB/C）小鼠被铜绿假单胞菌 AB030 株感染，并使用两种噬菌体 AP025 和 AP006 进行治疗。
结果表明，高浓度的噬菌体单剂量，细菌：噬菌体 1:10 和 1:100 有效消除血流感染并实现 100%小鼠存活。
这项研究强调了使用噬菌体单剂量使小鼠从菌血症中恢复的疗效。
