Anticancer Effects of -Derived Outer Membrane Vesicles Against Colorectal Cancer.

BACKGROUND

One of the deadliest cancers in the world, colorectal cancer has a dismal prognosis and a poor response to therapy. It was suggested that outer membrane vesicles (OMVs) produced by are a powerful inducer of inflammation in intestinal epithelial cells. This research aimed to determine the anticancer potential of -derived OMVs using a colorectal cancer model.

METHODS

Five distinct strains were collected for this study. Their OMVs were then isolated and characterized using dynamic light scattering (DLS) and scanning electron microscopy (SEM). The effects of -derived OMVs on colorectal cancer were evaluated in vitro and in vivo using a colorectal tumor model in nude mice.

RESULTS

Obtained results showed that probiotic strains released spherical-shaped vesicles ranging from 5 to 200 nm. -derived OMVs showed that in the untreated group, a large portion of the tumor tissue continued to grow, with only a few cells undergoing apoptosis. Conversely, the OMV-treated group exhibited a higher number of apoptotic cells, highlighting the anticancer effects of E. coli-derived OMVs in colorectal cancer.

CONCLUSIONS

These results demonstrated that -derived OMVs can be employed as a potential treatment for colorectal cancer with minimal adverse effects. Mechanistic studies indicate that these vesicles may promote apoptosis and inhibit cell proliferation, supporting their therapeutic potential.