Ghiyasvand Marjan, Hesampour Ardeshir, Dabiri Hossein, Arasteh Javad
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Microbiology Department, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Rep Biochem Mol Biol. 2024 Oct;13(3):438-444. doi: 10.61186/rbmb.13.3.438.
One of the deadliest cancers in the world, colorectal cancer has a dismal prognosis and a poor response to therapy. It was suggested that outer membrane vesicles (OMVs) produced by are a powerful inducer of inflammation in intestinal epithelial cells. This research aimed to determine the anticancer potential of -derived OMVs using a colorectal cancer model.
Five distinct strains were collected for this study. Their OMVs were then isolated and characterized using dynamic light scattering (DLS) and scanning electron microscopy (SEM). The effects of -derived OMVs on colorectal cancer were evaluated in vitro and in vivo using a colorectal tumor model in nude mice.
Obtained results showed that probiotic strains released spherical-shaped vesicles ranging from 5 to 200 nm. -derived OMVs showed that in the untreated group, a large portion of the tumor tissue continued to grow, with only a few cells undergoing apoptosis. Conversely, the OMV-treated group exhibited a higher number of apoptotic cells, highlighting the anticancer effects of E. coli-derived OMVs in colorectal cancer.
These results demonstrated that -derived OMVs can be employed as a potential treatment for colorectal cancer with minimal adverse effects. Mechanistic studies indicate that these vesicles may promote apoptosis and inhibit cell proliferation, supporting their therapeutic potential.
结直肠癌是世界上最致命的癌症之一，预后不佳且对治疗反应较差。有人提出，[具体细菌名称]产生的外膜囊泡（OMVs）是肠道上皮细胞炎症的有力诱导剂。本研究旨在使用结直肠癌模型确定[具体细菌名称]来源的OMVs的抗癌潜力。
本研究收集了五种不同的[具体细菌名称]菌株。然后使用动态光散射（DLS）和扫描电子显微镜（SEM）分离并表征它们的OMVs。使用裸鼠结直肠癌模型在体外和体内评估[具体细菌名称]来源的OMVs对结直肠癌的影响。
获得的结果表明，[具体细菌名称]益生菌菌株释放出5至200纳米的球形囊泡。[具体细菌名称]来源的OMVs显示，在未治疗组中，大部分肿瘤组织继续生长，只有少数细胞发生凋亡。相反，OMV治疗组显示出更多的凋亡细胞，突出了大肠杆菌来源的OMVs在结直肠癌中的抗癌作用。
这些结果表明，[具体细菌名称]来源的OMVs可作为结直肠癌的潜在治疗方法，且副作用最小。机制研究表明，这些囊泡可能促进凋亡并抑制细胞增殖，支持它们的治疗潜力。
