You Yimin, Li Shitong, Wang Liran, Zhao Xiyang, Zhang Dan, Chu Shaohua, Yang Xijia, Zhou Pei
Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural University, Changchun, China.
Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai, China.
Front Microbiol. 2025 Apr 22;16:1543933. doi: 10.3389/fmicb.2025.1543933. eCollection 2025.
Microbial remediation technology has the characteristics of high efficiency and environmental protection, which has attracted attention. However, there is complexity in the microorganism-soil-plant system. The effects of microbial agents on soil nutrients, plant quality, rhizosphere, and endophytic microorganisms are still unclear. Here, we demonstrate the application of NCT-2 as a multifunctional agent that concurrently addresses salinization-driven nutrient imbalances and reshapes keystone microbial taxa to restore soil-plant homeostasis. The results showed that NCT-2 agent improved the soil nutrients, reduced the loss of nitrogen and sulfur, increased the content of available phosphorus, and decreased the electrical conductivity. The agent increased the number of bacteria and fungi in the soil. Meanwhile, NCT-2 agent improved the vegetable quality and yield. Specifically, the NCT-2 agent significantly increased the aboveground fresh weight, underground fresh weight, total flavonoids, antioxidant enzyme activity, ascorbic acid, Cu, Zn, Fe, P, and K in lettuce, while significantly reduced nitrate. The chlorophyll a, chlorophyll b, carotenoids, and total chlorophyll were significantly increased by the agent. Critically, high-throughput sequencing revealed NCT-2-driven enrichment of stress-resilient taxa (e.g., , ) and functional synergists (e.g., ), which correlated with soil nutrient fluxes and plant antioxidant capacity. By decoupling the interplay between microbial community restructuring and systemic remediation outcomes, this work establishes a novel framework for leveraging keystone taxa to optimize salinized agroecosystems.
微生物修复技术具有高效、环保的特点，已受到关注。然而，微生物-土壤-植物系统存在复杂性。微生物制剂对土壤养分、植物品质、根际和内生微生物的影响仍不明确。在此，我们展示了NCT-2作为一种多功能制剂的应用，它能同时解决盐渍化导致的养分失衡问题，并重塑关键微生物类群以恢复土壤-植物的稳态。结果表明，NCT-2制剂改善了土壤养分，减少了氮和硫的流失，增加了有效磷含量，并降低了电导率。该制剂增加了土壤中细菌和真菌的数量。同时，NCT-2制剂提高了蔬菜品质和产量。具体而言，NCT-2制剂显著增加了生菜的地上鲜重、地下鲜重、总黄酮、抗氧化酶活性、抗坏血酸、铜、锌、铁、磷和钾的含量，同时显著降低了硝酸盐含量。该制剂还显著增加了叶绿素a、叶绿素b、类胡萝卜素和总叶绿素的含量。至关重要的是，高通量测序显示NCT-2驱动了抗逆类群（如 ， ）和功能协同菌（如 ）的富集，这与土壤养分通量和植物抗氧化能力相关。通过解开微生物群落重组与系统修复结果之间的相互作用，这项工作建立了一个利用关键类群优化盐渍化农业生态系统的新框架。
