Wang Bin, Zhang Dan, Chu Shaohua, Zhi Yuee, Liu Xiaorui, Zhou Pei
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Int J Genomics. 2020 Feb 28;2020:4109186. doi: 10.1155/2020/4109186. eCollection 2020.
NCT-2 is a nitrate-uptake bacterial, which shows high bioremediation capacity in secondary salinization soil, including nitrate-reducing capacity, phosphate solubilization, and salinity adaptation. To gain insights into the bioremediation capacity at the genetic level, the complete genome sequence was obtained by using a multiplatform strategy involving HiSeq and PacBio sequencing. The NCT-2 genome consists of a circular chromosome of 5.19 Mbp and ten indigenous plasmids, totaling 5.88 Mbp with an average GC content of 37.87%. The chromosome encodes 5,606 genes, 142 tRNAs, and 53 rRNAs. Genes involved in the features of the bioremediation in secondary salinization soil and plant growth promotion were identified in the genome, such as nitrogen metabolism, phosphate uptake, the synthesis of organic acids and phosphatase for phosphate-solubilizing ability, and Trp-dependent IAA synthetic system. Furthermore, strain NCT-2 has great ability of adaption to environments due to the genes involved in cation transporters, osmotic stress, and oxidative stress. This study sheds light on understanding the molecular basis of using NCT-2 in bioremediation of the secondary salinization soils.
NCT-2是一种硝酸盐吸收细菌，在次生盐渍化土壤中表现出较高的生物修复能力，包括硝酸盐还原能力、解磷能力和盐分适应性。为了从基因层面深入了解其生物修复能力，采用了包括HiSeq和PacBio测序在内的多平台策略获得了完整的基因组序列。NCT-2基因组由一条5.19 Mbp的环状染色体和十个内源质粒组成，总计5.88 Mbp，平均GC含量为37.87%。染色体编码5606个基因、142个tRNA和53个rRNA。在基因组中鉴定出了与次生盐渍化土壤生物修复特性和促进植物生长相关的基因，如氮代谢、磷吸收、解磷能力的有机酸和磷酸酶合成以及色氨酸依赖性吲哚-3-乙酸合成系统。此外，由于参与阳离子转运、渗透胁迫和氧化胁迫的基因，菌株NCT-2具有很强的环境适应能力。本研究为理解利用NCT-2进行次生盐渍化土壤生物修复的分子基础提供了线索。
