Shen Junxi, Wu Yi, Fang Leyao, Tan Zhoujin
School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Hanpu Science and Education Park, Yuelu District, Changsha, Hunan 410208 People's Republic of China.
3 Biotech. 2025 Jun;15(6):150. doi: 10.1007/s13205-025-04324-3. Epub 2025 May 3.
This study investigates the intestinal microecological mechanisms of Huoxiang Zhengqi (HXZQ) and its potential clinical applications in alleviating gastrointestinal (GI) disorders caused by cold and humid exposure. In this study, an animal model of cold exposure with GI disorders was prepared by simulating cold and humid environmental stress (CHS). Using this model, PacBio HiFi sequencing of intestinal mucosa full-length 16S rRNA and LC-MS targeted amino acid metabolomic analysis were conducted. An interaction network between the two was constructed to assess the microecological mechanism of intervention by HXZQ. Results indicate that HXZQ accelerates the recovery of GI disorders and restores the integrity of the mucus barrier in CHS mice. PacBio HiFi full-length sequencing of intestinal mucosa suggested that HXZQ can regulate the homeostasis of intestinal mucosal microbiota in CHS mice by promoting the proliferation of probiotics such as (with a 6% increase in relative abundance) and inhibiting conditional pathogenic bacteria such as (with its relative abundance reduced to 0%). Moreover, the integrated profiling of the microbiota amino acid metabolic function and LC/MS targeted amino acid indicated that Glu and Asp are the main metabolic pathways of HXZQ intervention by intestinal mucosal microbiota in CHS mice, which is significantly associated with based on the interaction network of intestinal mucosal microbiota and amino acid metabolism ( < 0.05). In conclusion, HXZQ plays a crucial role in maintaining intestinal mucosal microbiota homeostasis. By modulating the composition structure of intestinal mucosal microbiota, particularly , it facilitates the restoration of Glu and Asp amino acid metabolism in the host. These effects collectively contribute to the treatment of gastrointestinal disorders induced by cold and humid environmental stress.
The online version contains supplementary material available at 10.1007/s13205-025-04324-3.
本研究探讨藿香正气（HXZQ）的肠道微生态机制及其在缓解寒冷潮湿暴露引起的胃肠道（GI）疾病方面的潜在临床应用。在本研究中，通过模拟寒冷潮湿环境应激（CHS）制备了伴有胃肠道疾病的寒冷暴露动物模型。利用该模型，对肠道黏膜全长16S rRNA进行PacBio HiFi测序，并进行LC-MS靶向氨基酸代谢组分析。构建两者之间的相互作用网络，以评估HXZQ的干预微生态机制。结果表明，HXZQ可加速胃肠道疾病的恢复，并恢复CHS小鼠黏液屏障的完整性。肠道黏膜的PacBio HiFi全长测序表明，HXZQ可通过促进益生菌如（相对丰度增加6%）的增殖和抑制条件致病菌如（其相对丰度降至0%）来调节CHS小鼠肠道黏膜微生物群的稳态。此外，微生物群氨基酸代谢功能的综合分析和LC/MS靶向氨基酸表明，Glu和Asp是CHS小鼠肠道黏膜微生物群对HXZQ干预的主要代谢途径，基于肠道黏膜微生物群与氨基酸代谢的相互作用网络，这与显著相关（<0.05）。总之，HXZQ在维持肠道黏膜微生物群稳态中起关键作用。通过调节肠道黏膜微生物群的组成结构，特别是，它有助于宿主中Glu和Asp氨基酸代谢的恢复。这些作用共同有助于治疗寒冷潮湿环境应激引起的胃肠道疾病。
在线版本包含可在10.1007/s13205-025-04324-3获取的补充材料。
