College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China.
Department of Pediatrics, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China.
Microb Cell Fact. 2024 Jan 24;23(1):33. doi: 10.1186/s12934-024-02307-2.
Growing evidence has demonstrated that cold and humid environmental stress triggers gastrointestinal (GI) disorders. In this study, we explored the effects of intestinal microbiota homeostasis on the intestinal mucus barrier and GI disorders by cold and humid environmental stress. Moreover, the inner link between the intestinal mucosal microbiota and metabolites in mice with cold and humid environmental stress was interpreted by integrative analysis of PacBio HiFi sequencing microbial genomics and targeted metabolomics. In the current study, we found (1) after the cold and wet cold and humid environmental stress intervened in the intestinal microbiota disorder and homeostasis mice respectively, the bacterial culturing and fluorescein diacetate (FDA) microbial activity detection of intestinal microbiota including feces, intestinal contents, and intestinal mucosa suggested that the cold and humid environmental stress decreased the colony of culturable bacteria and microbial activity, in which intestinal microbiota disorder aggravated the injury of the intestinal mucus barrier and the GI symptoms related to cold and humid environmental stress; (2) the serum amino acid transferases such as glutamate pyruvic transa (GPT), and glutamic oxaloacetic transaminase (GOT) in cold and humid environmental stressed mice increased significantly, indicating that the intestinal microbiota adapted to cold and humid environmental stress by regulating the host's amino acid metabolism; (3) the integrative analysis of multi-omics illustrated a prediction model based on the microbiota Lactobacillus reuteri abundance and host amino acid level that can predict intestinal mucoprotein Muc2 with an adjusted R2 of 75.0%. In conclusion, the cold and humid environmental stress regulates the neurotransmitter amino acids metabolic function both in intestinal mucosal microbiota and host serum by adjusting the composition of the dominant bacterial population Lactobacillus reuteri, which contributes to the intestinal mucus barrier injury and GI disorders caused by cold and humid environmental stress.
越来越多的证据表明，寒冷和潮湿的环境应激会引发胃肠道（GI）疾病。在这项研究中，我们通过寒冷和潮湿的环境应激来探索肠道微生物组稳态对肠道黏液屏障和 GI 疾病的影响。此外，通过整合PacBio HiFi 测序微生物基因组学和靶向代谢组学分析，解释了寒冷和潮湿环境应激小鼠中肠道黏膜微生物群和代谢物之间的内在联系。在目前的研究中，我们发现：（1）在肠道微生物群紊乱和稳态小鼠分别接受寒冷和潮湿的环境应激干预后，粪便、肠内容物和肠黏膜中的肠道微生物群的细菌培养和荧光二乙酸（FDA）微生物活性检测表明，寒冷和潮湿的环境应激降低了可培养细菌的菌落和微生物活性，其中肠道微生物群紊乱加剧了肠道黏液屏障的损伤和与寒冷和潮湿环境应激相关的 GI 症状；（2）寒冷和潮湿环境应激小鼠的血清谷氨酸丙酮酸转氨酶（GPT）和谷草转氨酶（GOT）等氨基酸转移酶显著升高，表明肠道微生物群通过调节宿主的氨基酸代谢来适应寒冷和潮湿的环境应激；（3）多组学的综合分析说明了一个基于微生物群 Lactobacillus reuteri 丰度和宿主氨基酸水平的预测模型，可以预测肠道粘蛋白 Muc2，调整后的 R2 为 75.0%。总之，寒冷和潮湿的环境应激通过调节优势细菌种群 Lactobacillus reuteri 的组成，调节肠道黏膜微生物群和宿主血清中的神经递质氨基酸代谢功能，从而导致寒冷和潮湿环境应激引起的肠道黏液屏障损伤和 GI 疾病。
