Aliaga-Gálvez Romina, Gutiérrez Mario Felipe, Valenzuela Benjamín, Geraldeli Saulo, Abuna Gabriel, Inostroza Carolina, Bravo Cristian, Cochinski Gabriel, Loguercio Alessandro D
J Adhes Dent. 2025 May 7;27:103-114. doi: 10.3290/j.jad.c_2014.
This study aims to develop and characterize copper-doped bioactive glass nanoparticles (BG/CuNp), and to evaluate the effects of their addition into a resin composite on antimicrobial activity (AMA), cytotoxicity (CTX), ultimate tensile strength (UTS), Knoop microhardness (KHN), as well as immediate resin-dentin microtensile bond strength (μTBS), nanoleakage (NL) and in-situ degree of conversion (DC).
BG/CuNp were added to a resin composite at different concentrations (0% [control]; 5, 10 and 20 wt%). The AMA was evaluated against Streptococcus mutans. For CTX, the Gingival mesenchymal stem cells (GMSC) cell line was used. For UTS and KHN, specimens were tested after 24 h and 28 days. For bonding evaluation, a universal adhesive was applied on flat dentin surfaces, experimental resin composite build-ups were prepared, and specimens were sectioned to obtain resin-dentin sticks. These were evaluated for μTBS, NL and DC after water storage. Data were submitted to statistical analyses (α = 0.05).
The addition of 5% and 10% of BG/CuNp increases AMA (P 0.05), while the CTX remained unchanged with resin-containing BG/CuNp (P > 0.05). UTS and KHN remained stable with the addition of 5% and 10% of BG/CuNp at 24 h, but showed significantly higher values compared to the control after 28 d (P 0.05). μTBS and in-situ DC remained unchanged with BG/CuNp addition, regardless of the concentration added. However, significantly lower NL was observed for BG/CuNp groups (P 0.05).
The addition of BG/CuNp in the tested concentrations into a resin composite may be an alternative to provide antimicrobial activity and improve the integrity of the hybrid layer, without compromising biological, adhesives and mechanical properties.
本研究旨在开发和表征铜掺杂生物活性玻璃纳米颗粒（BG/CuNp），并评估将其添加到树脂复合材料中对抗菌活性（AMA）、细胞毒性（CTX）、极限拉伸强度（UTS）、努氏显微硬度（KHN）以及即时树脂-牙本质微拉伸粘结强度（μTBS）、纳米渗漏（NL）和原位转化率（DC）的影响。
将BG/CuNp以不同浓度（0%[对照组]；5%、10%和20%重量比）添加到树脂复合材料中。评估其对变形链球菌的AMA。对于CTX，使用牙龈间充质干细胞（GMSC）细胞系。对于UTS和KHN，在24小时和28天后对标本进行测试。对于粘结评估，在平坦的牙本质表面应用通用粘结剂，制备实验性树脂复合材料堆积物，并将标本切片以获得树脂-牙本质棒。在水储存后对这些标本进行μTBS、NL和DC评估。数据进行统计分析（α = 0.05）。
添加5%和10%的BG/CuNp可提高AMA（P < 0.05），而含BG/CuNp的树脂复合材料的CTX保持不变（P > 0.05）。在24小时时，添加5%和10%的BG/CuNp后UTS和KHN保持稳定，但在28天后与对照组相比显示出显著更高的值（P < 0.05）。添加BG/CuNp后，无论添加浓度如何，μTBS和原位DC保持不变。然而，BG/CuNp组的NL显著更低（P < 0.05）。
将测试浓度的BG/CuNp添加到树脂复合材料中可能是一种提供抗菌活性并改善混合层完整性的替代方法，而不会损害生物学、粘结和机械性能。
