Fan Qianjin, Huangfu Haoyue, Chen Lan, Jiao Mengqi, Li Beijie, Cao Zhijie, Sun Hui, Luo Xuelian, Xu Jianguo
Institute of Public Health, School of Medicine, Nankai University, Tianjin 300071, China.
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Int J Mol Sci. 2025 Apr 8;26(8):3500. doi: 10.3390/ijms26083500.
Enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease (HFMD), leading to a serious health threat to young children. Probiotics are effective at treating or preventing gastrointestinal infections, especially viral infections. Probiotics against EV71 are mainly traditional lactic acid-producing bacteria, and most of them have been proven to be effective only in vitro. Here, we report that the marine bacterium X-1125 () has promising anti-EV71 activity. The antiviral effect of against EV71 was assessed in different cell lines, and the viral RNA levels and titers were obviously reduced after treatment with . Furthermore, we established an EV71-infected mouse model to evaluate its antiviral efficacy in vivo. The oral administration of significantly decreased the viral loads in the hindlimb muscles, spleens, and ileums. Further research revealed that enhances the expression of type I interferon (IFN-I) in EV71-infected cells. Similarly, transcriptome analysis indicated that the expression of interferon-stimulated genes (ISGs) in EV71-infected mice significantly increased after treatment. Taken together, the results of this study indicated that markedly reduces EV71 infection by regulating the IFN response both in vivo and in vitro, providing a potential means to work against EV71 infection.
肠道病毒71型（EV71）是手足口病（HFMD）的主要病原体，对幼儿的健康构成严重威胁。益生菌在治疗或预防胃肠道感染，尤其是病毒感染方面具有疗效。针对EV71的益生菌主要是传统的产乳酸菌，其中大多数仅在体外被证明有效。在此，我们报道海洋细菌X-1125（）具有可观的抗EV71活性。在不同细胞系中评估了其对EV71的抗病毒作用，用处理后，病毒RNA水平和滴度明显降低。此外，我们建立了EV71感染的小鼠模型以评估其体内抗病毒功效。口服显著降低了后肢肌肉、脾脏和回肠中的病毒载量。进一步研究表明，可增强EV71感染细胞中I型干扰素（IFN-I）的表达。同样，转录组分析表明，处理后，EV71感染小鼠中干扰素刺激基因（ISGs）的表达显著增加。综上所述，本研究结果表明，通过在体内和体外调节IFN反应，可显著降低EV71感染，为对抗EV71感染提供了一种潜在手段。
