Zhang Tingting, Yu Yinchen, Li Jianyu, Zheng Li, Chen Shiwei, Mao Jianjun
School of Advanced Manufacturing, Fuzhou University, Jinjiang 362251, China.
Jinjiang City Fuzhou University Science and Education Park Development Center, Fuzhou University, Jinjiang 362251, China.
Insects. 2025 Apr 3;16(4):380. doi: 10.3390/insects16040380.
Artificial diets applied in the mass-rearing propagation of can improve reproductive ability by optimizing the feeding formula. This study used transcriptome data to investigate the effects of various artificial diets on the growth and development of . Results indicate that spawning increased with the low-fat and JH III-supplemented artificial diet (Diet 3). Furthermore, the highest glycogen content found in Diet 3 was significantly different from the other two groups. Triglyceride content decreased as adult feeding time increased in the three artificial diet groups, with the fastest decrease observed in the low-fat diet (Diet 2). Protein content increased gradually in the high-fat diet (Diet 1) group compared to the other treatment groups. The adults reared on low-fat artificial diets, when compared to those on artificial diets supplemented with juvenile hormones at the transcriptome level, were found to have upregulated genes enriched in ubiquitin-mediated proteolysis, ribosome biogenesis, and the hedgehog signaling pathway. In contrast, the genes upregulated in the latter group were enriched in oxidative phosphorylation, amino acid biosynthesis, and the metabolism of other amino acids. The results suggest that nutritional status significantly affects the growth and development of and has practical implications for the artificial feeding of natural pest enemies.
应用于大规模饲养繁殖的人工饲料可通过优化喂养配方提高繁殖能力。本研究利用转录组数据研究了不同人工饲料对其生长发育的影响。结果表明，补充低脂和保幼激素III的人工饲料（饲料3）可提高产卵量。此外，饲料3中的糖原含量最高，与其他两组有显著差异。在三种人工饲料组中，随着成虫取食时间的增加，甘油三酯含量下降，其中低脂饲料（饲料2）下降最快。与其他处理组相比，高脂饲料（饲料1）组的蛋白质含量逐渐增加。在转录组水平上，与补充保幼激素的人工饲料饲养的成虫相比，低脂人工饲料饲养的成虫上调的基因富集于泛素介导的蛋白水解、核糖体生物发生和刺猬信号通路。相反，后一组上调的基因富集于氧化磷酸化、氨基酸生物合成和其他氨基酸代谢。结果表明，营养状况显著影响其生长发育，对天然害虫天敌的人工饲养具有实际意义。
