Vaswani K K
Life Sci. 1985 Sep 23;37(12):1107-15. doi: 10.1016/0024-3205(85)90354-6.
Effects of neonatal thiamine deficiency and vitamin A deficiency on total and fractions of gangliosides (GT1, GD1a, GD1b and GM1) were studied in Charles Foster rat brain at 21 days of age. GT1, GD1b+GD1a and GM1 are being presented here as poly-, di- and mono-sialo gangliosides. Thiamine and vitamin A deficiencies were induced by feeding mothers essentially thiamine and vitamin A free diets respectively. A normal control (G+L+) and weight matched undernourished groups (G+L- for thiamine and LL for vitamin A experiments) were used for comparison. At 21 days, the concentration of total gangliosides in thiamine deficient and G+L- rat brains were 49.0% and 45.7%; in vitamin A deficient and LL group were 66.6% and 88.0% of the G+L+ group, respectively. The percent contribution of poly-, di- and mono-sialo gangliosides in G+L+/thiamine deficient/G+L- were; 17.2/46.8/73.5, 54.4/51.7/14.2, and 6.6/8.7/5.8, respectively. The percent contribution of poly-, di- and mono-sialo gangliosides in G+L+/vitamin A deficient/LL were; 19.3/39.9/43.7, 57.0/37.6/35.1, and 8.4/11.6/19.7 respectively. The changes observed in these experiments suggest an underlying possibility of metabolic defect in undernourished animals.
研究了新生期硫胺素缺乏和维生素A缺乏对21日龄查尔斯·福斯特大鼠脑内神经节苷脂总量及各组分（GT1、GD1a、GD1b和GM1）的影响。此处将GT1、GD1b + GD1a和GM1分别呈现为多唾液酸、二唾液酸和单唾液酸神经节苷脂。分别通过给母鼠喂食基本不含硫胺素和维生素A的饲料来诱导硫胺素和维生素A缺乏。使用正常对照组（G + L +）以及体重匹配的营养不良组（硫胺素实验为G + L -，维生素A实验为LL）进行比较。在21日龄时，硫胺素缺乏组和G + L -组大鼠脑内神经节苷脂总量分别为G + L +组的49.0%和45.7%；维生素A缺乏组和LL组分别为G + L +组的66.6%和88.0%。G + L + /硫胺素缺乏组/G + L -组中多唾液酸、二唾液酸和单唾液酸神经节苷脂的贡献百分比分别为：17.2/46.8/73.5、54.4/51.7/14.2和6.6/8.7/5.8。G + L + /维生素A缺乏组/LL组中多唾液酸、二唾液酸和单唾液酸神经节苷脂的贡献百分比分别为：19.3/39.9/43.7、57.0/37.6/35.1和8.4/11.6/19.7。这些实验中观察到的变化表明营养不良动物存在潜在的代谢缺陷可能性。
