Xia Fengming, He Haojiang, Ma Jize, Jin Yutian, Qiao Qing, Long Peng, Li Ping, Sun Rui
College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China.
Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China.
Molecules. 2025 Apr 8;30(8):1663. doi: 10.3390/molecules30081663.
Lacquer trees are an important economic tree species in China, and raw lacquer is its main secondary metabolite. Polyphenolic compounds are the primary components of raw lacquer, among which diene urushiol exhibits high inhibitory activity against the reverse transcriptase of acquired immunodeficiency syndrome (AIDS). Therefore, this study established and optimized the ultrasound-assisted extraction process of diene urushiol from lacquer tree leaves. Based on single-factor experiments on the number of extractions, extraction time, extraction temperature, and solvent to solid ratio, the Box-Behnken Design response surface methodology was employed to obtain the optimal extraction process, which included three extractions, an extraction time of 55 min, an extraction temperature of 50 °C, and a solvent to solid ratio of 10:1 mL/g. Under these conditions, the content of diene urushiol was 4.56 mg/g (FW), which bore no significant difference from the theoretical value of 4.69 mg/g (FW), indicating a good model fit. Therefore, response surface methodology (RSM) can be used to optimize the extraction process of diene urushiol from lacquer leaves. This method lays a solid foundation for the comprehensive development and utilization of lacquer tree resources.
漆树是我国重要的经济树种，生漆是其主要次生代谢产物。多酚类化合物是生漆的主要成分，其中二烯漆酚对获得性免疫缺陷综合征（艾滋病）逆转录酶具有较高的抑制活性。因此，本研究建立并优化了从漆树叶中超声辅助提取二烯漆酚的工艺。在对提取次数、提取时间、提取温度和料液比进行单因素实验的基础上，采用Box-Behnken设计响应面法获得了最佳提取工艺，即提取3次，提取时间55分钟，提取温度50℃，料液比10:1 mL/g。在此条件下，二烯漆酚含量为4.56 mg/g（鲜重），与理论值4.69 mg/g（鲜重）无显著差异，表明模型拟合良好。因此，响应面法（RSM）可用于优化漆树叶中二烯漆酚的提取工艺。该方法为漆树资源的综合开发利用奠定了坚实基础。
