Wang Teng, Zou Xiaolin, Zhang Hong, Li Jiwen, Peng Xiaoming, Ju Ruijun, Jia Zhaojun, Wen Zhenguo, Li Cuiqing
Department of Pharmaceutics, Beijing Institute of Petrochemical Technology, Beijing 102627, China.
College of Science and Technology, Hebei Agricultural University, Huanghua 061100, China.
Molecules. 2025 Apr 14;30(8):1747. doi: 10.3390/molecules30081747.
In this study, an ultrasonic-assisted procedure for the extraction of mulberry leaf polysaccharides (MLPs) was investigated using response surface methodology with a 29-run Box-Behnken design. Four factors were investigated, and it was found that the factors influencing the process, in order of significance, were the extraction temperature > liquid-to-material ratio > ultrasonic power. Considering practical conditions, the parameters were adjusted to a liquid-to-material ratio of 16:1 mL/g, extraction time of 58 min, extraction temperature of 65 °C, and ultrasonic power of 500 W. Under these conditions, the yield of MLPs was 14.47%, which is close to the predicted value, indicating that the extraction process optimized by response surface methodology (RSM) is feasible. The separation and purification effects of macroporous resin and activated carbon on MLPs were investigated, with the D152 resin being considered the most suitable choice. The optimal separation conditions were found to be a sample concentration of 0.5 g/mL and an optimal flow rate of 1 mL/min. Thin-layer chromatography and infrared spectroscopy revealed that polysaccharides extracted from mulberry leaves are primarily composed of rhamnose, xylose, and arabinose. In conclusion, this study successfully optimized the ultrasonic-assisted extraction process of MLPs through response surface methodology, determined the optimal parameter combination, and verified its efficiency and stability. Under the optimal conditions obtained for ultrasonic-assisted extraction, the yield of MLPs is significantly higher than that reported in the previous literature. The decolorization process of crude mulberry leaf polysaccharide extract was also investigated, and the purified MLPs have clear monosaccharide composition and structural characteristics, providing a theoretical basis and technical support for their application in functional food or drug development.
在本研究中，采用响应面法和29次运行的Box-Behnken设计，研究了超声辅助提取桑叶多糖（MLPs）的工艺。研究了四个因素，发现影响该工艺的因素按重要性排序为：提取温度＞液料比＞超声功率。考虑实际条件，将参数调整为液料比16:1 mL/g、提取时间58分钟、提取温度65℃、超声功率500W。在此条件下，MLPs的得率为14.47%，接近预测值，表明通过响应面法（RSM）优化的提取工艺是可行的。研究了大孔树脂和活性炭对MLPs的分离纯化效果，认为D152树脂是最合适的选择。最佳分离条件为样品浓度0.5 g/mL，最佳流速1 mL/min。薄层色谱和红外光谱显示，从桑叶中提取的多糖主要由鼠李糖、木糖和阿拉伯糖组成。总之，本研究通过响应面法成功优化了MLPs的超声辅助提取工艺，确定了最佳参数组合，并验证了其效率和稳定性。在超声辅助提取获得的最佳条件下，MLPs的得率显著高于以往文献报道。还研究了粗桑叶多糖提取物的脱色工艺，纯化后的MLPs具有明确的单糖组成和结构特征，为其在功能性食品或药物开发中的应用提供了理论依据和技术支持。
