Wu Mengna, Liu Zijun, Wang Jiahui, Hu Wentao, Zhang Huawei
School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
College of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, China.
Molecules. 2025 Apr 8;30(8):1657. doi: 10.3390/molecules30081657.
An Arctic marine-derived strain, MNP-1, was characterized by a combined methodological approach, incorporating a variety of analytical techniques including morphological features, biochemical characteristics, and 16S ribosomal RNA (rRNA) sequence analysis. The chemical investigation of sp. MNP-1 using the OSMAC (one strain many compounds) strategy yielded the isolation of twenty known compounds (), which were unambiguously identified by various spectroscopic approaches including H and C NMR and ESI-MS (previously reported data). Bioassay results indicated that compounds , , , , , , and had antimicrobial activity against human pathogenic strains including , , and with MIC values ranging from 4 to 32 μg/mL, and compounds and exhibited moderate inhibitory activity on A549, MCF-7, and HepG2 tumor lines showing IC values within the range of 19.88 to 35.82 µM. These findings suggest that sp. MNP-1 is one of the prolific manufacturers of bioactive secondary metabolites with therapeutic potential.
一株源自北极海洋的菌株MNP - 1通过综合方法进行了表征，该方法结合了多种分析技术，包括形态特征、生化特性和16S核糖体RNA（rRNA）序列分析。使用OSMAC（一种菌株多种化合物）策略对MNP - 1菌株进行化学研究，分离出了20种已知化合物（），通过包括氢谱和碳谱核磁共振以及电喷雾电离质谱（先前报道的数据）在内的各种光谱方法对其进行了明确鉴定。生物测定结果表明，化合物，，，，，，和对包括，和在内的人类致病菌株具有抗菌活性，最低抑菌浓度值范围为4至32μg/mL，化合物和对A549、MCF - 7和HepG2肿瘤细胞系表现出中等抑制活性，半数抑制浓度值在19.88至35.82μM范围内。这些发现表明，MNP - 1菌株是具有治疗潜力的生物活性次级代谢产物的多产制造商之一。
