Kim Eun-Sook, Jeong Ji-Eon, Kim Young-Hoi, You Yong-Ouk
Institute of Biomaterials and Implant, College of Dentistry, Wonkwang University, Iksan 54538, Republic of Korea.
Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan 54538, Republic of Korea.
Molecules. 2025 Apr 14;30(8):1755. doi: 10.3390/molecules30081755.
The purpose of this study was to evaluate the gene expression pattern of the caries-inhibiting effect of () extract on cariogenic bacteria (). We examined bacterial growth, tooth surface attachment, biofilm formation, acid production, free calcium release, and toxicity gene expression. The major components of the extract were investigated by UPLC-Q-TOF-MS analysis. Exposure to inhibited bacterial growth and attachment at concentrations of ≥15 μg/mL. Inhibition effects on biofilm formation, acid production, and free calcium release due to acid production were observed at concentrations ≥ 30 μg/mL. virulence gene expression analysis showed that it inhibited the expression of and , which mediate bacterial attachment to the tooth surface, and that of genes contributing to biofilm formation (, , and ) and acid resistance ( and ), and regulation (). Analysis using UPLC-Q-TOF-MS/MS showed that the main component was phenylpropanoids. These results suggest that may inhibit the cariogenic properties associated with the expression of caries-related genes in .
本研究的目的是评估（）提取物对致龋菌（）的防龋作用的基因表达模式。我们检测了细菌生长、牙齿表面附着、生物膜形成、产酸、游离钙释放和毒性基因表达。通过超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）分析研究了提取物的主要成分。暴露于≥15μg/mL浓度的（提取物）可抑制细菌生长和附着。在≥30μg/mL浓度时，观察到对生物膜形成、产酸以及产酸导致的游离钙释放的抑制作用。（致龋菌）毒力基因表达分析表明，它抑制了介导细菌附着于牙齿表面的（相关基因）以及和的表达，以及对生物膜形成（、和）、耐酸性（和）和调节（相关基因）有贡献的基因的表达。使用UPLC-Q-TOF-MS/MS分析表明主要成分是苯丙素类化合物。这些结果表明（提取物）可能抑制与（致龋菌）中龋相关基因表达相关的致龋特性。
