Institute of Biomaterials and Implant, College of Dentistry, Wonkwang University, Iksan 54538, Republic of Korea.
Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan 54538, Republic of Korea.
Molecules. 2024 Oct 16;29(20):4907. doi: 10.3390/molecules29204907.
This study aimed to identify the inhibitory effect of essential oil (AREO) on the cariogenic properties of , which causes dental caries and dental plaque formation. After extracting the AREO, their effects on the growth and acid production of were examined. Furthermore, biofilm formation was observed on the resin teeth surface. The effect on the expression of biofilm-related genes of was measured using real-time PCR. AREO components were analyzed using gas chromatography (GC) and GC-mass spectrometry (MS). The growth and acid production of were significantly inhibited at concentrations of 0.02 mg/mL or higher of AREO. At 0.04 mg/mL, inhibition was similar to that of the positive control, 0.1% NaF. AREO suppressed the expression of virulence factors such as , , , , , , , and at concentrations of 0.02 mg/mL or higher. As a result of GC and GC-MS analyses, the main components of AREO included estragole, limonene, and β-caryophyllene. These results suggest that may be a useful agent for inhibiting the cariogenic properties of .
本研究旨在确定精油（AREO）对可致龋的 的抑制作用， 可导致龋齿和牙菌斑形成。提取 AREO 后，检测其对 的生长和产酸的影响。此外，观察树脂牙齿表面的生物膜形成。使用实时 PCR 测量对生物膜相关基因表达的影响。使用气相色谱（GC）和 GC-质谱（MS）分析 AREO 成分。在浓度为 0.02 毫克/毫升或更高的 AREO 下， 的生长和产酸明显受到抑制。在 0.04 毫克/毫升时，抑制作用与阳性对照 0.1%氟化钠相似。AREO 在 0.02 毫克/毫升或更高的浓度下抑制毒力因子如 、 、 、 、 、 、和 的表达。通过 GC 和 GC-MS 分析，AREO 的主要成分包括莪术烯、柠檬烯和 β-石竹烯。这些结果表明，AREO 可能是抑制 的致龋特性的有用剂。
