Yin Xiulian, You Qinghong, Jiang Zhonghai, Zhou Xinghai
School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223003, China.
School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223003, China; Jiangsu Key Laboratory of Quality Control and Further Processing of Cereals and Oils, Nanjing University of Finance and Economics, 301 Wenyuan Road, Nanjing 210023, China.
Int J Biol Macromol. 2016 Feb;83:226-32. doi: 10.1016/j.ijbiomac.2015.11.059. Epub 2015 Dec 2.
Ultrasonic-microwave synergistic extraction (UMSE) of polysaccharides from Cornus officinalis was optimized by response surface methodology (RSM). The effect of four different factors on the yield of C. officinalis polysaccharides (COP) was studied. RSM results showed that the optimal conditions were extraction time of 31.49823 min, microwave power of 99.39769 W, and water-to-raw material ratio of 28.16273. The COP yield was 11.38±0.31% using the modified optimal conditions, which was consistent with the value predicted by the model. The crude COP was purified by DEAE-Cellulose 52 chromatography and Sephadex G-100 chromatography. Five fractions, namely, crude COP, COP-1, COP-2, COP-3, and COP-4, were obtained. Monosaccharide composition analysis revealed that the COP was composed of glucose, arabinose, fucose, xylose, mannose, and rhamnose. Preliminary structural characterizations of COP were conducted by scanning electron microscopy and Fourier transform infrared spectroscopy.
采用响应面法（RSM）对山茱萸多糖的超声-微波协同提取（UMSE）工艺进行了优化。研究了四个不同因素对山茱萸多糖（COP）得率的影响。RSM结果表明，最佳条件为提取时间31.49823分钟、微波功率99.39769瓦、料液比28.16273。采用修正后的最佳条件，COP得率为11.38±0.31%，与模型预测值一致。粗COP经DEAE-纤维素52柱色谱和Sephadex G-100柱色谱纯化。得到了五个级分，即粗COP、COP-1、COP-2、COP-3和COP-4。单糖组成分析表明，COP由葡萄糖、阿拉伯糖、岩藻糖、木糖、甘露糖和鼠李糖组成。通过扫描电子显微镜和傅里叶变换红外光谱对COP进行了初步结构表征。
