Yang Wuhao, Yuan Hui, Sun Hao, Hu Jiangshan, Xu Yaping, Li Yuhang, Qiu Yan
College of Materials, Xiamen University, Xiamen 361005, China.
State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
Molecules. 2025 Apr 20;30(8):1848. doi: 10.3390/molecules30081848.
Chronic wound healing remains a major challenge in diabetes management due to prolonged inflammation, autonomic neuropathy, and bacterial infections. In particular, multidrug-resistant bacterial infections are important to the development of diabetic wounds, leading to persistent inflammation and delayed healing. To address this issue, we developed a self-adaptive nanozyme designed to modulate infectious and inflammatory microenvironments by doping Ce and Ag into mesoporous silicon nanomaterials (MSNs). The resulting CA@MSNs exhibited strong bacterial capture capabilities via electrostatic attraction. Additionally, the synergistic effects of Ce and Ag endowed CA@MSNs with peroxidase (POD)-like activity, enabling the generation of reactive oxygen species (ROS) to eradicate bacteria in infectious microenvironments. Notably, CA@MSNs also demonstrated the ability to scavenge a broad spectrum of ROS, including hydroxyl free radicals, hydrogen peroxide, and superoxide radicals, in inflammatory microenvironments. This dual functionality helped mitigate inflammation and promote endothelial cell migration. Consequently, treatment with CA@MSNs significantly reduced inflammation, enhanced fibroblast activation, and facilitated collagen deposition, ultimately accelerating the healing of methicillin-resistant (MRSA)-infected wounds in diabetic mice. In conclusion, this study presents a promising therapeutic strategy for chronic diabetic wounds, offering a novel approach to overcoming infection-related healing delays.
由于炎症持续时间长、自主神经病变和细菌感染，慢性伤口愈合仍然是糖尿病管理中的一项重大挑战。特别是，多重耐药细菌感染对糖尿病伤口的发展至关重要，会导致持续炎症和愈合延迟。为了解决这个问题，我们开发了一种自适应纳米酶，通过将铈（Ce）和银（Ag）掺杂到介孔硅纳米材料（MSNs）中来调节感染性和炎症性微环境。由此产生的CA@MSNs通过静电吸引表现出强大的细菌捕获能力。此外，Ce和Ag的协同作用赋予CA@MSNs类似过氧化物酶（POD）的活性，能够产生活性氧（ROS）以根除感染性微环境中的细菌。值得注意的是，CA@MSNs还表现出在炎症性微环境中清除多种ROS的能力，包括羟基自由基、过氧化氢和超氧自由基。这种双重功能有助于减轻炎症并促进内皮细胞迁移。因此，用CA@MSNs治疗可显著减轻炎症、增强成纤维细胞活化并促进胶原蛋白沉积，最终加速糖尿病小鼠耐甲氧西林金黄色葡萄球菌（MRSA）感染伤口的愈合。总之，本研究为慢性糖尿病伤口提供了一种有前景的治疗策略，为克服与感染相关的愈合延迟提供了一种新方法。
