Queen J Edal, Prasad T Augustine Arul, Vithiya B Scholastica Mary, Odhah Omalsad H, Kumar Nadavala Siva, Tamizhdurai P, Alreshaidan Salwa B, Basivi Praveen Kumar, Pabba Durga Prasad, Al-Fatesh Ahmed S
PG and Research Department of Chemistry, Dwaraka Doss Goverdhan Doss Vaishnav College (Affiliated to University of Madras), Arumbakkam, Chennai 600106, Tamil Nadu, India.
PG and Research Department of Chemistry, Dwaraka Doss Goverdhan Doss Vaishnav College (Affiliated to University of Madras), Arumbakkam, Chennai 600106, Tamil Nadu, India.
Bioorg Chem. 2025 Jul 1;161:108546. doi: 10.1016/j.bioorg.2025.108546. Epub 2025 May 4.
The ever-growing field of nanotechnology requires optimized green manufacturing of gold nanoparticles. The current study focuses to optimize the green synthesis of gold nanoparticles (AuNPs) using cranberry fruit extract, which has a rich phytochemical content and can operate as a reducing agent. The UV-Vis spectrum indicated the absorption at 530-550nm, which is characteristic of gold nanoparticles in the bottom-up approach. The IR spectra provided valuable information about the phytochemicals present in the plant extract. The major peaks of AuNPs in XRD and SEM, TEM images affirmed the synthesized nanoparticles are spherical in shape. The thermal stability of the nanoparticles was exemplified using TG-DTA analysis. Further, the synthesized particles were employed to study in-vitro cytotoxicity activity against MCF-7 breast cancerous cells and at its highest concentration (200 mL), the AuNPs showed highest cytotoxicity activity of 90.43 % against MCF-7 cell lines. The Anti-bacterial activity of the synthesized nanoparticles was observed on both gram positive and gram negative pathogens where the gram-negative pathogens such as Klebsiella pneumoniae and Pseudomonas aeruginosa showed highest zone of inhibition (21 mm and 23 mm), while gram positive pathogens such as Bacillus subtilis and Enterococcus faecalis showed less zone of inhibition (17 mm and 15 mm). Additionally, Response Surface Methodology (RSM) was utilized to optimize the average diameter size of AuNPs using the Box Behnken design (BBD). The bio synthesized AuNPs are also found effective in degradation of Methylene Bule (MB), an electrophilic dye and Metanil Yellow (MY), an anionic azo dye. Overall, this study displays that cranberry-mediated AuNPs can be a sustainable substitute in biological and environmental applications, in addition to adding to the expanding corpus of research on green nanotechnology.
不断发展的纳米技术领域需要优化金纳米颗粒的绿色制造。当前的研究重点是利用蔓越莓果实提取物优化金纳米颗粒（AuNPs）的绿色合成，该提取物含有丰富的植物化学成分，可作为还原剂。紫外可见光谱表明在530 - 550nm处有吸收，这是自下而上方法中金纳米颗粒的特征。红外光谱提供了有关植物提取物中存在的植物化学成分的有价值信息。XRD以及SEM、TEM图像中AuNPs的主要峰证实合成的纳米颗粒呈球形。使用TG - DTA分析举例说明了纳米颗粒的热稳定性。此外，将合成颗粒用于研究对MCF - 7乳腺癌细胞的体外细胞毒性活性，并在其最高浓度（200 μL）下，AuNPs对MCF - 7细胞系显示出90.43%的最高细胞毒性活性。在革兰氏阳性和革兰氏阴性病原体上均观察到合成纳米颗粒的抗菌活性，其中革兰氏阴性病原体如肺炎克雷伯菌和铜绿假单胞菌显示出最高的抑菌圈（21mm和23mm），而革兰氏阳性病原体如枯草芽孢杆菌和粪肠球菌显示出较小的抑菌圈（17mm和15mm）。此外，利用响应面方法（RSM）通过Box Behnken设计（BBD）优化AuNPs的平均直径大小。还发现生物合成的AuNPs对亲电染料亚甲基蓝（MB）和阴离子偶氮染料甲基橙黄（MY）具有有效的降解作用。总体而言，这项研究表明，蔓越莓介导的AuNPs除了增加绿色纳米技术的研究范围外，还可以在生物和环境应用中成为一种可持续的替代品。
