Zhang Xiaobo, Wang Xiuwen, Zhu Longjiao, Zhu Jinyan, Zheng Qiuyue, Yuan Jingli, Xu Wentao, Cao Jijuan
Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, School of Life Sciences, Dalian Minzu University, Dalian, 116600, China.
Food Laboratory of Zhongyuan, Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing, 100193, China.
Biosens Bioelectron. 2025 Sep 1;283:117558. doi: 10.1016/j.bios.2025.117558. Epub 2025 May 5.
Salmonella typhimurium (S. typhimurium), as common and highly pathogenic foodborne pathogen, poses a significant risk to public safety worldwide. The development of highly sensitive, rapid and on-site method for S. typhimurium analysis is urgently needed to prevent bacterial infections. Herein, we introduced a CRISPR/Cas12a-driven electrochemiluminescence (ECL) sensor based on luminol/O binary systems for S. typhimurium detection, employing ultrafine Pt nanoclusters-anchored 2D delaminated-MXenes (Pt NCs/D-MXenes) as the co-reactant accelerator. The ultrathin D-MXenes support regulates the size and dispersibility of Pt NCs and facilitates the full exposure of active sites, and synergistic interactions between D-MXenes and Pt NCs improves electrocatalytic properties toward the reduction of O, which promotes the generation of ROS for boosting ECL emission. Using target responsive-regulated CRISPR/Cas12a system, the ECL sensor for S. typhimurium showed a broad concentration range from 10 to 10 CFU/mL and limit of detection of 6 CFU/mL, with satisfactory recoveries in spiked-actual samples.
鼠伤寒沙门氏菌作为一种常见且致病性很强的食源性病原体，对全球公共安全构成了重大风险。迫切需要开发一种高灵敏度、快速且可现场使用的方法来分析鼠伤寒沙门氏菌，以预防细菌感染。在此，我们介绍了一种基于鲁米诺/O二元体系的CRISPR/Cas12a驱动的电化学发光（ECL）传感器，用于检测鼠伤寒沙门氏菌，采用超细铂纳米簇锚定的二维分层MXenes（Pt NCs/D-MXenes）作为共反应促进剂。超薄的D-MXenes载体调节了Pt NCs的尺寸和分散性，并促进了活性位点的充分暴露，并且D-MXenes与Pt NCs之间的协同相互作用改善了对O还原的电催化性能，从而促进了活性氧的生成以增强ECL发射。利用靶标响应调控的CRISPR/Cas12a系统，用于检测鼠伤寒沙门氏菌的ECL传感器显示出10至10 CFU/mL的宽浓度范围和6 CFU/mL的检测限，在加标实际样品中具有令人满意的回收率。
