Gulyaeva N V, Konoshenko G I, Mokhova E N
Membr Biochem. 1985;6(1):19-32. doi: 10.3109/09687688509065440.
A lipophilic fluorescent cation diS-C3-(5) and rotenone suppress the oxygen consumption rate of thymocytes in similar concentrations. Seventy percent inhibition corresponds to an inhibitor:cytochrome a molar ratio of about 1:1. Addition of uncouplers decreases the inhibition of respiration by diS-C3-(5) (but not rotenone). FCCP in similar concentrations increases O2 consumption in the absence of diS-C3-(5) and the diS-C3-(5) fluorescence intensity in the presence of TMPD in thymocyte suspensions. In most thymocyte preparations, oligomycin (0.05-0.1 microgram/mL) increases the fluorescence of diS-C3-(5) and further addition of TMPD (50-100 microM) decreases the fluorescence. Addition of NaCN (400 microM) after oligomycin leads to a fluorescence increase that is hardly affected by subsequent addition of 0.2 microM FCCP. Nigericin (10-50 nM) decreases the diS-C3-(5) fluorescence. The data indicate that the diS-C3-(5) fluorescence associated with mitochondrial transmembrane potential (delta psi m) may be an essential part of the diS-C3-(5) fluorescence in lymphocyte suspensions. The changes of the diS-C3-(5) fluorescence intensity in the presence of TMPD after FCCP addition reflect delta psi m.
亲脂性荧光阳离子二硫代-C3-(5)和鱼藤酮在相似浓度下可抑制胸腺细胞的氧消耗率。70%的抑制率对应抑制剂与细胞色素a的摩尔比约为1:1。加入解偶联剂可降低二硫代-C3-(5)对呼吸的抑制作用（但鱼藤酮不会）。相似浓度的羰基氰化物-对三氟甲氧基苯腙（FCCP）在无二硫代-C3-(5)时可增加氧消耗，在胸腺细胞悬液中存在四甲基对苯二胺（TMPD）时可增加二硫代-C3-(5)的荧光强度。在大多数胸腺细胞制剂中，寡霉素（0.05 - 0.1微克/毫升）可增加二硫代-C3-(5)的荧光，进一步加入TMPD（50 - 100微摩尔）可降低荧光。在寡霉素之后加入氰化钠（400微摩尔）会导致荧光增加，随后加入0.2微摩尔FCCP对其影响不大。尼日利亚菌素（10 - 50纳摩尔）可降低二硫代-C3-(5)的荧光。数据表明，与线粒体跨膜电位（Δψm）相关的二硫代-C3-(5)荧光可能是淋巴细胞悬液中二硫代-C3-(5)荧光的重要组成部分。加入FCCP后在TMPD存在下二硫代-C3-(5)荧光强度的变化反映了Δψm。
