Mokhova E N, Rozovskaya I A
J Bioenerg Biomembr. 1986 Aug;18(4):265-76. doi: 10.1007/BF00743047.
The effects of uncouplers (FCCP, DNF), oligomycin, and rotenone on the fluorescence of potential-sensitive dyes, rhodamine 123 and diS-C3-(5), in lymphocyte suspensions were compared. The fluorescence of these optical probes gradually increased at higher FCCP concentrations. The dependences of fluorescence intensities and FCCP concentrations were similar for both dyes, and only diS-C3-(5) fluorescence started increasing at lower FCCP concentrations. Rotenone (1 microM) significantly increased rhodamine 123 fluorescence. TMPD-induced and uncoupler-induced diS-C3-(5) fluorescence changes increased 1.5- to 2-fold if the incubation mixture was supplemented with oligomycin (0.1-0.2 microgram/ml). The fluorescence responses of the dyes in the lymphocyte suspension correlate with the effects of mitochondrial energetics inhibitors on delta psi m in isolated mitochondria. The results suggest the possibility of using these dyes for estimating the direction of the delta psi m changes in the lymphocyte suspension.
比较了解偶联剂（FCCP、DNF）、寡霉素和鱼藤酮对淋巴细胞悬液中电位敏感染料罗丹明123和二硫代氰基荧光素（diS-C3-(5)）荧光的影响。在较高的FCCP浓度下，这些光学探针的荧光逐渐增加。两种染料的荧光强度与FCCP浓度的依赖性相似，且只有diS-C3-(5)的荧光在较低的FCCP浓度下开始增加。鱼藤酮（1微摩尔）显著增加罗丹明123的荧光。如果在孵育混合物中添加寡霉素（0.1 - 0.2微克/毫升），四甲基对苯二胺诱导的和解偶联剂诱导的diS-C3-(5)荧光变化增加1.5至2倍。染料在淋巴细胞悬液中的荧光反应与线粒体能量学抑制剂对分离线粒体中Δψm的影响相关。结果表明使用这些染料估计淋巴细胞悬液中Δψm变化方向的可能性。
