Yoh M, Honda T, Miwatani T, Uemura T, Kuwata H, Fukai K
Microbiol Immunol. 1985;29(5):413-9. doi: 10.1111/j.1348-0421.1985.tb00842.x.
For detection of Legionella pneumophila and determination of its serogroup, the modified staphylococcal coagglutination test was studied in detail. Cross-reactions in serum-agglutination tests were observed among serogroups, but immunoglobulin-coated staphylococcal cells could detect L. pneumophila with high sensitivity (a cell concentration with an absorbance of 0.008 at 660 nm could be detected) and serogroups could be determined without cross-reactions. Moreover the coexistence of other bacteria did not affect the results of the test. These results suggest that the staphylococcal coagglutination test is useful for detection and identification of Legionella, especially in environmental samples, and for serogrouping of isolates of L. pneumophila from clinical specimens.
为检测嗜肺军团菌及其血清群，对改良葡萄球菌凝聚试验进行了详细研究。在血清凝集试验中观察到血清群之间存在交叉反应，但免疫球蛋白包被的葡萄球菌细胞能够高灵敏度地检测嗜肺军团菌（可检测到在660nm处吸光度为0.008的细胞浓度），并且能够在无交叉反应的情况下确定血清群。此外，其他细菌的共存并不影响试验结果。这些结果表明，葡萄球菌凝聚试验对于嗜肺军团菌的检测和鉴定（尤其是在环境样本中）以及对临床标本中分离出的嗜肺军团菌进行血清分型很有用。
