Para M F, Plouffe J F
J Clin Microbiol. 1983 Oct;18(4):895-900. doi: 10.1128/jcm.18.4.895-900.1983.
To better define the surface antigens of Legionella pneumophila for clinical and experimental purposes, we have produced monoclonal antibodies to L. pneumophila serogroups 1 and 6. Two hybridomas were produced in serogroup 1. One antibody, LP-I-17, recognized a serogroup-common antigen. The second antibody, LP-I-81, was specific for serogroup 1. This antibody was able to agglutinate bacterial cells belonging to the serogroup 1 reference strains. Philadelphia and Knoxville. Microagglutination assays of environmental and clinical isolates revealed a subgroup of serogroup 1 environmental isolates which were not agglutinated by LP-I-81. This subset of isolates was segregated to certain buildings in the medical complex. Immunodiffusion studies showed identity between the LP-I-81 antigen and the serogroup-specific antigen of serogroup 1 organisms. This antigen could be absorbed out of the serogroup 1 organism extract with LP-I-81-coated Staphylococcus aureus, leaving the serogroup-common antigens. Three hybridomas were produced to serogroup 6. All three produced antibodies which were serogroup 6 specific and agglutinated serogroup 6 bacteria.
为了更好地确定嗜肺军团菌的表面抗原用于临床和实验目的，我们制备了针对嗜肺军团菌血清型1和6的单克隆抗体。在血清型1中产生了两种杂交瘤。一种抗体LP-I-17识别一种血清型共同抗原。第二种抗体LP-I-81对血清型1具有特异性。该抗体能够凝集属于血清型1参考菌株费城株和诺克斯维尔株的细菌细胞。对环境和临床分离株的微量凝集试验显示，血清型1环境分离株中有一个亚组不能被LP-I-81凝集。这一分离株亚组分布在医疗综合大楼的某些建筑物中。免疫扩散研究表明，LP-I-81抗原与血清型1菌株的血清型特异性抗原相同。这种抗原可以用包被有LP-I-81的金黄色葡萄球菌从血清型1菌株提取物中吸收出来，留下血清型共同抗原。针对血清型6产生了三种杂交瘤。所有三种杂交瘤产生的抗体均对血清型6具有特异性，并能凝集血清型6细菌。
