Magdy Wasfy Reham, Abdoulaye Anissa, Borentain Patrick, Mbaye Babacar, Tidjani Alou Maryam, Caputo Aurelia, Andrieu Claudia, Mottola Giovanna, Levasseur Anthony, Million Matthieu, Gerolami Rene
IHU Méditerranée Infection, Marseille, France.
MEPHI, Aix-Marseille Université, Marseille, France.
Gut Pathog. 2025 May 7;17(1):27. doi: 10.1186/s13099-025-00703-6.
Gut microbiota alteration is implicated in the pathogenesis of alcoholic liver disease (ALD) and associated hepatocellular carcinoma (HCC). No study has characterized the dysbiosis associated with ALD by microbial culturomics, which certifies viability and allows pathobiont strain candidates to be characterized.
A case-control study (n = 59) was conducted on patients with ALD without HCC (ALD-NoHCC, n = 16), ALD with HCC (ALD-HCC, n = 19) and controls (n = 24) groups. 16 S rRNA amplicon sequencing and microbial culturomics were used as complementary methods for gut microbiome profiling.
Compared to the control group, Thomasclavelia ramosa and Gemmiger formicilis were significantly increased in the ALD-HCC group and Mediterraneibacter gnavus was significantly increased in the ALD-NoHCC group using 16 S rRNA sequencing. By microbial culturomics, T. ramosa was detected in all ALD samples (100%), and the most enriched since cultivated in only a small proportion of controls (20%, p < 0.001).
T. ramosa, identified by culturomics and 16 rRNA sequencing, may be associated with ALD and ALD-HCC. These results highlight the potential role of T. ramosa in liver cancer, in line with its genotoxic properties and its tumor growth-promoting effect in gnotobiotic mice recently reported.
肠道微生物群改变与酒精性肝病（ALD）及相关肝细胞癌（HCC）的发病机制有关。尚无研究通过微生物培养组学对与ALD相关的菌群失调进行特征描述，微生物培养组学可证实微生物的生存能力，并对潜在致病菌株进行特征描述。
对无HCC的ALD患者（ALD-无HCC组，n = 16）、伴有HCC的ALD患者（ALD-HCC组，n = 19）和对照组（n = 24）进行病例对照研究（n = 59）。16S rRNA扩增子测序和微生物培养组学被用作肠道微生物组分析的互补方法。
使用16S rRNA测序，与对照组相比，在ALD-HCC组中，纤细托马斯菌和甲酸吉米菌显著增加，在ALD-无HCC组中，食窦栖热菌显著增加。通过微生物培养组学，在所有ALD样本中均检测到纤细托马斯菌（100%），且由于其仅在一小部分对照组中培养（20%，p < 0.001），因此最为富集。
通过培养组学和16 rRNA测序鉴定出的纤细托马斯菌可能与ALD和ALD-HCC有关。这些结果突出了纤细托马斯菌在肝癌中的潜在作用，这与其遗传毒性特性以及最近报道的在无菌小鼠中的促肿瘤生长作用一致。
