Togo Amadou Hamidou, Diop Awa, Bittar Fadi, Maraninchi Marie, Valero René, Armstrong Nicholas, Dubourg Grégory, Labas Noémie, Richez Magali, Delerce Jeremy, Levasseur Anthony, Fournier Pierre-Edouard, Raoult Didier, Million Matthieu
Aix Marseille Univ, IRD, MEPHI, IHU-Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005, Marseille, France.
Aix Marseille Univ, IRD, VITROME, IHU-Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005, Marseille, France.
Antonie Van Leeuwenhoek. 2018 Nov;111(11):2107-2128. doi: 10.1007/s10482-018-1104-y. Epub 2018 May 31.
An anaerobic isolate, strain AT7, was cultivated from a stool sample of a morbidly obese French woman using a microbial culturomics approach. The 16S rRNA gene sequence analysis showed that strain AT7 exhibited 96% nucleotide sequence similarity with Ruminococcus torques strain JCM 6553 (= ATCC 27756 = VPI B2-51), currently the closest related species with a validly published name. The strain was observed to be a Gram-stain positive, non-motile, asporogenous and coccobacillary-shaped bacterium. It was found to be catalase positive and oxidase negative. Its major fatty acids were identified as C (54%) and C (30%). The draft genome of strain AT7 is 3,069,882 bp long with 42.4% G+C content. 2925 genes were predicted, including 2867 protein-coding genes and 58 RNAs. Based on phenotypic, biochemical, phylogenetic and genomic evidence, we propose the creation of the new genus Mediterraneibacter and species, Mediterraneibacter massiliensis, that contains strain AT7 (= CSUR P2086 = DSM 100837), and the reclassification of Ruminococcus faecis, Ruminococcus lactaris, Ruminococcus torques, Ruminococcus gnavus, Clostridium glycyrrhizinilyticum as Mediterraneibacter faecis comb. nov., with type strain Eg2 (= KCTC 5757 = JCM15917), Mediterraneibacter lactaris comb. nov., with type strain ATCC 29176 (= VPI X6-29), Mediterraneibacter torques comb. nov., with type strain ATCC 27756 (= VPI B2-51), Mediterraneibacter gnavus comb. nov., with type strain ATCC 29149T (= VPI C7-9T) and Mediterraneibacter glycyrrhizinilyticus comb. nov., with type strain ZM35 (= JCM 13368 = DSM 17593), respectively.
采用微生物培养组学方法，从一名病态肥胖法国女性的粪便样本中培养出一株厌氧分离株AT7。16S rRNA基因序列分析表明，菌株AT7与瘤胃球菌属扭矩瘤胃球菌菌株JCM 6553（=ATCC 27756=VPI B2-51）的核苷酸序列相似性为96%，后者是目前已有效发表名称的最密切相关物种。观察到该菌株为革兰氏染色阳性、无运动性、无芽孢且呈球杆菌状的细菌。发现其过氧化氢酶阳性，氧化酶阴性。其主要脂肪酸被鉴定为C（54%）和C（30%）。菌株AT7的基因组草图长3,069,882 bp，G+C含量为42.4%。预测有2925个基因，包括2867个蛋白质编码基因和58个RNA。基于表型、生化、系统发育和基因组证据，我们提议创建新属地中海杆菌属及新物种马西利亚地中海杆菌，该属包含菌株AT7（=CSUR P2086=DSM 100837），并将粪便瘤胃球菌、乳酸瘤胃球菌、扭矩瘤胃球菌、纤细瘤胃球菌、甘草酸解梭菌分别重新分类为粪便地中海杆菌新组合，模式菌株为Eg2（=KCTC 5757=JCM15917），乳酸地中海杆菌新组合，模式菌株为ATCC 29176（=VPI X6-29），扭矩地中海杆菌新组合，模式菌株为ATCC 27756（=VPI B2-51），纤细地中海杆菌新组合，模式菌株为ATCC 29149T（=VPI C7-9T），甘草酸解地中海杆菌新组合，模式菌株为ZM35（=JCM 13368=DSM 17593）。
