Skalka M, Cejková M
Mol Biol Rep. 1985 Apr;10(3):163-8. doi: 10.1007/BF00778523.
The action of 1,10-phenanthroline-copper complex under aerobic conditions in the presence of 2-mercaptoethanol on chromatin DNA in murine thymocyte nuclei was studied. At limited oxygen supply primarily multiple single-strand breaks of DNA in the spacer segments are observed while the core DNA segments in chromatin remain intact. After the single-strand breaks accumulate in both DNA strands (under conditions of improved oxygen supply), double-strand cleavage of DNA to fragments of nucleosomal size becomes apparent. This regular character of DNA degradation in chromatin is apparently due to the preferential binding of the 1,10-phenanthroline-copper complex to DNA of the spacer segments and to the localized generation of damaging radicals.
研究了在有氧条件下，1,10-菲咯啉-铜配合物在2-巯基乙醇存在时对小鼠胸腺细胞核染色质DNA的作用。在氧气供应有限时，主要观察到间隔区DNA出现多个单链断裂，而染色质中的核心DNA片段保持完整。在两条DNA链中都积累了单链断裂后（在氧气供应改善的条件下），DNA双链断裂成核小体大小的片段变得明显。染色质中DNA降解的这种规律性显然是由于1,10-菲咯啉-铜配合物优先与间隔区的DNA结合以及损伤自由基的局部产生。
