Poznanovic G, Sevaljevic L
Mol Biol Rep. 1984 Jan;9(4):231-4. doi: 10.1007/BF00775353.
Incubation of rat liver nuclei with the o-phenantroline-CuSO4 (OP-Cu) complex under conditions not causing any DNA cleavage, enhanced the susceptibility of chromatin to the action of micrococcal nuclease. The released nucleosomal fraction had less coextracted nonhistone proteins, while the nuclear matrix was enriched in nonhistone proteins when compared with the controls. These changes were interpreted as the consequence of a displacement of nonhistone proteins from their closer association with the chromatin complex and a concomitant exposure of chromatin regions in a state less protected by nonhistone proteins.
在不会导致任何DNA切割的条件下，将大鼠肝细胞核与邻菲罗啉 - 硫酸铜（OP-Cu）复合物一起温育，增强了染色质对微球菌核酸酶作用的敏感性。与对照相比，释放的核小体部分共提取的非组蛋白较少，而核基质中的非组蛋白则有所富集。这些变化被解释为非组蛋白从与染色质复合物的紧密结合中被取代，以及染色质区域在较少受非组蛋白保护的状态下伴随暴露的结果。
