An increase of the regulating factor for chromatin-dependent RNA polymerase II reactions in the tightly-bound chromatin fraction by glucocorticoid injection.

A sugar-containing factor for chromatin-dependent RNA polymerase II reactions exists in rat liver nuclei (1). In this communication the localization of this factor in cell nuclei was investigated. The major activity of the factor was observed in the nuclear soluble fraction whereas a minor activity was detected in the tightly-bound chromatin fraction, but not in the loosely bound chromatin fraction. The factor in the tightly-bound chromatin fraction was considerably increased by glucocorticoid injection, but not in other fractions.