Horikoshi M, Sekimizu K, Natori S
J Biol Chem. 1984 Jan 10;259(1):608-11.
The interaction between RNA polymerase II and its specific stimulatory factor S-II was studied using each component purified to near homogeneity. It was found that RNA polymerase II and S-II form a complex in the absence of DNA and nucleoside triphosphates, although their interaction was weak. This interaction became stable in the presence of a template and substrates, and a stoichiometric amount of S-II was found to remain in the transcription machinery during RNA chain elongation.
利用纯化至接近均一性的各组分，对RNA聚合酶II与其特异性刺激因子S-II之间的相互作用进行了研究。结果发现，在不存在DNA和核苷三磷酸的情况下，RNA聚合酶II和S-II形成复合物，尽管它们之间的相互作用较弱。在存在模板和底物的情况下，这种相互作用变得稳定，并且发现在RNA链延伸过程中，化学计量的S-II保留在转录机制中。
