Saini Jyoti, Bakshi Jaimanti, Panda Naresh Kumar, Sharma Maryada, Yadav Ashok Kumar, Narayansami Suruthy, Goyal Atul Kumar
Department of Otolaryngology and Head & Neck Surgery, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Indian J Surg Oncol. 2025 Apr;16(2):601-610. doi: 10.1007/s13193-024-02113-7. Epub 2024 Oct 23.
Oral cancer is one of most common noncommunicable diseases that imposed significant burden on healthcare around the globe including India. Several molecular markers have been extensively researched for their potential as diagnostic and prognostic biomarkers for oral cancer. Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) found to play a crucial role in cytoskeleton dynamics and thus tumor growth and metastasis. The present study aims to analyze the MMP (MMP-1, MMP-3, MMP-9, and MMP-13) and TIMP (TIMP-1 and TIMP-3) expression in the oral cancer patients as compared to healthy controls. We also aim to compare the diagnostic accuracy of MMPs and TIMPs on enzyme-linked immunosorbent assay (ELISA) with respect to the immunohistochemistry (IHC). A total of 15 oral cancer patients and 14 healthy controls were enrolled in this study. Blood sample from oral cancer patients and healthy controls was obtained to analyze the MMP and TIMP expression levels in serum sample using ELISA. Tumor tissue sample from patients for IHC analysis was obtained by biopsy under local anesthesia. Data were compared between ELISA and IHC for sensitivity and specificity analysis. The mean age of the patients was 47.07 ± 15.74 years with the highest incidence of disease found in male gender (86.7%). MMPs and TIMPs exhibited higher expression in oral cancer patients as compared to healthy controls and the difference was statistically significant in case of MMP-13. MMPs and TIMPs showed stronger expression at advanced stages of cancer on IHC with MMP-9 exhibiting the highest expression level. The highest sensitivity and specificity on ELISA with respect to IHC were exhibited by the MMP-3 followed by MMP-13, TIMP-1, TIMP-3, and MMP-1. MMP-3 and MMP-13 were found to be reliable in terms of diagnostic marker for oral cancer as they found to have the highest sensitivity and specificity on ELISA with respect to IHC. MMP-9 was found to be reliable as a prognostic marker as it expresses highest on IHC at advanced stages of oral cancer.
口腔癌是全球（包括印度）医疗保健领域负担沉重的最常见非传染性疾病之一。人们对几种分子标志物作为口腔癌诊断和预后生物标志物的潜力进行了广泛研究。基质金属蛋白酶（MMPs）及其组织抑制剂（TIMPs）在细胞骨架动态变化中发挥关键作用，进而影响肿瘤生长和转移。本研究旨在分析口腔癌患者与健康对照者中MMP（MMP-1、MMP-3、MMP-9和MMP-13）和TIMP（TIMP-1和TIMP-3）的表达情况。我们还旨在比较酶联免疫吸附测定（ELISA）和免疫组织化学（IHC）检测MMPs和TIMPs的诊断准确性。本研究共纳入15例口腔癌患者和14名健康对照者。采集口腔癌患者和健康对照者的血样，采用ELISA分析血清样本中MMP和TIMP的表达水平。通过局部麻醉下活检获取患者的肿瘤组织样本用于IHC分析。比较ELISA和IHC数据的敏感性和特异性。患者的平均年龄为47.07±15.74岁，男性发病率最高（86.7%）。与健康对照者相比，MMPs和TIMPs在口腔癌患者中表达更高，MMP-13的差异具有统计学意义。在IHC检测中，MMPs和TIMPs在癌症晚期表达更强，MMP-9表达水平最高。ELISA检测相对于IHC检测，MMP-3的敏感性和特异性最高，其次是MMP-13、TIMP-1、TIMP-3和MMP-1。MMP-3和MMP-13在口腔癌诊断标志物方面被认为是可靠的，因为它们在ELISA检测相对于IHC检测时具有最高的敏感性和特异性。MMP-9被认为是可靠的预后标志物，因为它在口腔癌晚期的IHC检测中表达最高。
