Waterhouse Dale J, Borsetto Daniele, Santarius Thomas, Tysome James R, Bohndiek Sarah E
University of Cambridge, Department of Physics, Cambridge, United Kingdom.
University of Cambridge, Cancer Research UK Cambridge Institute, Cambridge, United Kingdom.
J Biomed Opt. 2025 May;30(5):056002. doi: 10.1117/1.JBO.30.5.056002. Epub 2025 May 7.
The definitive treatment for pituitary adenoma is transsphenoidal surgical resection. Conventional white light imaging shows limited contrast between the adenoma and the pituitary gland, and only the tissue surface is visualized, leaving a pressing unmet need for improved intraoperative adenoma delineation to preserve pituitary function during surgery.
To evaluate the potential of multispectral imaging to enhance visualization of adenoma during transsphenoidal resection.
A multispectral camera based on a spectrally resolved detector array was coupled to a standard 4-mm rigid endoscope for imaging, such that the camera head could easily be switched with the standard of care camera head during surgery.
The multispectral imaging (MSI) endoscope was deployed during transsphenoidal surgery, and usable data were obtained from 12 patients. MSI was able to distinguish between an adenoma and a healthy pituitary based on the spectral angle with the reference spectrum of blood.
The MSI endoscope holds the potential to differentiate adenoma tissue and healthy pituitary. With further development, MSI endoscopy could enable real-time label-free delineation of tumors during surgery, based on quantitative thresholds, which should contribute to improving the completeness of resection, while helping to preserve the pituitary gland, preventing serious life-changing complications.
垂体腺瘤的确定性治疗方法是经蝶窦手术切除。传统的白光成像显示腺瘤与垂体之间的对比度有限，且仅能观察到组织表面，这使得在手术中改善腺瘤的术中轮廓以保留垂体功能的迫切需求仍未得到满足。
评估多光谱成像在经蝶窦切除术中增强腺瘤可视化的潜力。
将基于光谱分辨探测器阵列的多光谱相机与标准的4毫米刚性内窥镜耦合用于成像，以便在手术过程中相机头可以轻松地与标准护理相机头互换。
多光谱成像（MSI）内窥镜在经蝶窦手术中得到应用，从12例患者中获得了可用数据。MSI能够根据与血液参考光谱的光谱角度区分腺瘤和健康垂体。
MSI内窥镜具有区分腺瘤组织和健康垂体的潜力。随着进一步发展，MSI内窥镜可以基于定量阈值在手术期间实现肿瘤的实时无标记轮廓描绘，这应有助于提高切除的完整性，同时有助于保留垂体，预防严重的改变生活的并发症。
