Impact of oleuropein on and adhesion and its mediated toxicity in Zebrafish () embryos.

BACKGROUND AND OBJECTIVES

The rising prevalence of antibiotic resistance and biofilm-associated infections poses significant challenges in clinical settings. This study investigates the antimicrobial and anti-adhesive properties of oleuropein, a compound derived from olive leaves, against and

MATERIALS AND METHODS

This study was conducted on (fluconazole-resistant/susceptible) and (methicillin-resistant/susceptible). The antifungal, antibacterial, anti-adhesion, and cell surface hydrophobicity (CSH) effects of oleuropein were evaluated. The impact of oleuropein on germ tube formation (GTF) in was assessed. Finally, the toxicity of oleuropein was evaluated in zebrafish embryos.

RESULTS

Oleuropein exhibited MIC values of 10 mg/ml for and 5 mg/ml for . It significantly (P< 0.05) reduced the adhesion of both microorganisms in a dose-dependent manner, with inhibition percentages of 78.43% and 75.91% for and , respectively. Additionally, oleuropein reduced the CSH of , indicating its potential to interfere with adhesion mechanisms. In addition, oleuropein exhibited inhibition of GTF in

CONCLUSION

Oleuropein demonstrates significant antimicrobial and anti-adhesive properties against and indicating its potential as a therapeutic agent for preventing biofilm-related infections. However, careful dosage management is crucial due to its observed toxicity at higher concentrations.