Ebineshan Kumar, Srikantam Aparna, Pallapati Michael Sukumar
Department of Microbiology, LEPRA - Society, Blue Peter Public Health and Research Centre, Hyderabad, India.
Department of Microbiology, Malla Reddy Medical College for Women, Hyderabad, India.
Iran J Microbiol. 2025 Apr;17(2):211-219. doi: 10.18502/ijm.v17i2.18394.
is one of the predominant biofilm producing pathogen in leprosy foot ulcer (LFU). The objective of this study was to identify the transcriptome profile through Next Generation Sequencing (NGS) approach in mature biofilm of leprosy foot ulcer isolate of
A cross-sectional study was conducted from July 2019 to May 2022 and a total of twenty-seven isolates were collected from the foot ulcers of leprosy patients. All isolates were screened for biofilm formation in vitro. Initially, two potential biofilm producing isolates and two planktonic cells were selected for transcriptome comparison.
With reference to transcriptome profile, out of 2,842 genes, 2,688 genes in mature biofilm and 2,685 genes in planktonic cells were expressed. Among them, forty-five differentially expressed genes with 32 and 13 genes showing up and down regulation respectively were obtained.
The research emphasizes the need for continued exploration into the mechanisms of biofilm formation by , particularly in the context of leprosy foot ulcers. Understanding these pathways not only aids in grasping the complexity of chronic infections but also paves the way for innovative therapeutic approaches aimed at mitigating biofilm-related complications in clinical settings.
[病原体名称未给出]是麻风足溃疡（LFU）中主要的生物膜产生病原体之一。本研究的目的是通过下一代测序（NGS）方法确定麻风足溃疡分离株成熟生物膜中的转录组图谱。
2019年7月至2022年5月进行了一项横断面研究，共从麻风患者的足部溃疡中收集了27株分离株。对所有分离株进行体外生物膜形成筛选。最初，选择了两株潜在的生物膜产生分离株和两株浮游细胞进行转录组比较。
参照转录组图谱，在2842个基因中，成熟生物膜中有2688个基因表达，浮游细胞中有2685个基因表达。其中，获得了45个差异表达基因，分别有32个基因上调和13个基因下调。
该研究强调了继续探索[病原体名称未给出]生物膜形成机制的必要性，特别是在麻风足溃疡的背景下。了解这些途径不仅有助于理解慢性感染的复杂性，也为旨在减轻临床环境中生物膜相关并发症的创新治疗方法铺平了道路。
